PF3D7_1133400 apical membrane antigen 1

PfAAP6 (PF3D7_0508900), (B) PfAAP4 (PF3D7_1318700), (C) PfAAP2

(PF3D7_1312800): Schematic representation of each protein (protein length indicated as number of amino acids) on top. Localization of AAP-GFP fusion proteins in schizonts and free merozoites and PCR-based confirmation of the correct insertion of GFP-encoding integration plasmid into the targeted loci shown underneath. Ladder size indicated in base pairs (bp). gDNA from parental 3D7 was used as control. Nuclei stained with Hoechst-33342. Scale bars 2 μm. (D) Co-localization of PfAAP6-GFP with

the rhoptry bulb marker protein ARO-mCherry, the microneme marker AMA1-mCherry and the rhoptry neck marker AIP-mCherry. (E) Co-localization of PfAAP4-GFP with the rhoptry bulb marker protein ARO-mCherry, the microneme marker AMA1-mCherry, the rhoptry neck marker RON12-mCherry, the dense granules marker EXP2-mCherry and the IMC marker ALV5-mCherry. (F) Structured illumination microscopy (SIM) images of PfAAP4-GFP with the IMC marker ALV5-mCherry during IMC formation. Time points (T) indicated are T1-T3: early schizonts, T4-T6: late schizonts, and T7: free merozoites. Zoom factor: 400%. Scale bars 2 μm.

Wichers JS, Wunderlich J, Heincke D, Pazicky S, Strauss J, Schmitt M, Kimmel J, Wilcke L, Scharf S, von Thien H, Burda PC, Spielmann T, Löw C, Filarsky M, Bachmann A, Gilberger TW.Identification of novel inner membrane complex and apical annuli proteins of the malaria parasite Plasmodium falciparum. Cell Microbiol. 2021 Apr 8:e13341.

Other associated proteins

PFID Formal Annotation
PF3D7_0414900 armadillo-domain containing rhoptry protein
PF3D7_0508900 protein aap6
PF3D7_1003600 inner membrane complex protein 1c, putative
PF3D7_1017100 rhoptry neck protein 12
PF3D7_1312800 protein aap2, putative
PF3D7_1318700 protein aap4
PF3D7_1471100 exported protein 2