Upper panel: Colocalization of PfAtg18 with food vacuole markers. GFP/PfAtg18-expressing P. falciparum trophozoites cultured in the presence of lysotracker, and P. falciparum trophozoites co-expressing GFP/PfAtg18 and PfCRT/mCherry were assessed by live cell fluorescence microscopy. Panels from the left represent signal for lysotracker or PfCRT, GFP/PfAtg18 (Atg18), nucleic acid staining (Hoechst), bright field showing parasite and RBC boundaries (DIC), and the merge of all images (Merged). Both lysotracker and PfCRT overlap with GFP/PfAtg18, and also colocalize with the in-built food vacuole marker haemozoin. Right panel: Localization patterns of PfAtg18. Live or fixed trophozoites of GFP/PfAtg18-expressing parasites were evaluated for localization of PfAtg18 by live cell fluorescence microscopy (A) and confocal microscopy (B), respectively. The images are representative for PfAtg18 within the food vacuole (FV) and around the food vacuole membrane (FVM). Panels from the left represent signals for GFP/PfAtg18 (Atg18), nucleic acid staining (Nuc), bright field with parasite and RBC boundaries (DIC), and the merge of all images (Merged).
Sudhakar R, Das D, Thanumalayan S, Gorde S, Sijwali PS. Plasmodium falciparum Atg18 localizes to the food vacuole via interaction with the multi-drug resistance protein 1 and phosphatidylinositol 3-phosphate. Biochem J. 2021 12:BCJ20210001.
Other associated proteins
|PF3D7_0709000||chloroquine resistance transporter|