Proteins identified in IRBC-derived vesicles

Most-abundant proteins in RMVS

Shown are the top 20 parasite proteins ranked by peptide counts that were identified in RMVs derived from iRBCs, from both parasite strains analyzed. Among the most abundant proteins are parasite invasion ligands (EBA-175 and EBA-181) and RBC membrane-associated proteins (e.g., RhopH2/3, EXP-2, CLAG3.2, RESA, SBP1, and MAHRP). Measurements were made on ring stage parasites.

Mantel PY, Hoang AN, Goldowitz I, Potashnikova D, Hamza B, Vorobjev I, Ghiran I, Toner M, Irimia D, Ivanov AR, Barteneva N, Marti M. Malaria-infected erythrocyte-derived microvesicles mediate cellular communication within the parasite population and with the host immune system. Cell Host Microbe. 2013 May 15;13(5):521-534. PMID: 23684304

PfID

Annotation

Transcript

PF3D7_0929400

high molecular weight rhoptry protein 2

PF3D7_1246200

actin I

PF3D7_0302500

cytoadherence linked asexual protein 3.1

PF3D7_1471100

exported protein 2

PF3D7_0102500

erythrocyte binding antigen-181

PF3D7_0905400

high molecular weight rhoptry protein 3

PF3D7_0619400

cell division cycle protein 48 homologue, putative

PF3D7_0501300

skeleton-binding protein 1

PF3D7_0409600

replication protein A1, large subunit

PF3D7_0818900

heat shock protein 70

PF3D7_1357000

elongation factor 1-alpha

PF3D7_1116800

heat shock protein 101 chaperone protein ClpB2

PF3D7_0501600

rhoptry-associated protein 2

PF3D7_0220800

cytoadherence linked asexual protein 2

PF3D7_0501000

Plasmodium exported protein, unknown function

PF3D7_1462800

glyceraldehyde-3-phosphate dehydrogenase

1.2.1.12

PF3D7_1353200

membrane associated histidine-rich protein 2

PF3D7_1325900

conserved Plasmodium protein, unknown function

PF3D7_0102200

ring-infected erythrocyte surface antigen

Dekel E, Yaffe D, Rosenhek-Goldian I, Ben-Nissan G, Ofir-Birin Y, Morandi MI, Ziv T, Sisquella X, Pimentel MA, Nebl T, Kapp E, Ohana Daniel Y, Karam PA, Alfandari D, Rotkopf R, Malihi S, Temin TB, Mullick D, Revach OY, Rudik A, Gov NS, Azuri I, Porat Z, Bergamaschi G, Sorkin R, Wuite GJL, Avinoam O, Carvalho TG, Cohen SR, Sharon M, Regev-Rudzki N. 20S proteasomes secreted by the malaria parasite promote its growth. Nat Commun. 2021 12(1):1172. PMID: 33608523

PF3D7_0608500

proteasome subunit alpha type-2, putative

3.4.25.1

PF3D7_1470900

proteasome subunit beta type-2, putative

3.4.25.1

PF3D7_0108000

proteasome subunit beta type-3, putative

3.4.25.1

PF3D7_1328100

proteasome subunit beta type-7, putative

3.4.25.1

PF3D7_1223100

cAMP-dependent protein kinase regulatory subunit

2.7.11.11

PF3D7_1444500

eukaryotic initiation factor 2alpha kinase 1

PF3D7_1122800

calcium-dependent protein kinase 6

2.7.11.13

PF3D7_0724600

protein kinase, putative

2.7.11.1

PF3D7_0823000

serine/threonine protein kinase vps15

PF3D7_0615500

cdc2-related protein kinase 5

2.7.11.22

PF3D7_0718100

exported serine/threonine protein kinase

2.7.11.1

PF3D7_0628200

eukaryotic translation initiation factor 2-alpha kinase

2.7.11.1

PF3D7_0311400

kinase-related protein pkrp

PF3D7_0926300

protein kinase, putative

2.7.11.17

PF3D7_0211700

tyrosine kinase-like protein, putative

2.7.11.1

PF3D7_0826700

receptor for activated c kinase

PF3D7_0704500

serine/threonine protein kinase, putative

2.7.11.1

PF3D7_1441300

serine/threonine protein kinase, putative

2.7.11.1

Vimonpatranon S, Roytrakul S, Phaonakrop N, Lekmanee K, Atipimonpat A, Srimark N, Sukapirom K, Chotivanich K, Khowawisetsut L, Pattanapanyasat K. Extracellular Vesicles Derived from Early and Late Stage Plasmodium falciparum-Infected Red Blood Cells Contain Invasion-Associated Proteins. J Clin Med. 2022 11(14):4250. PMID: 35888014;