The proteome of the apicoplast
Boucher MJ, Ghosh S, Zhang L, Lal A, Jang SW, Ju A, Zhang S, Wang X, Ralph SA, Zou J, Elias JE, Yeh E. Integrative proteomics and bioinformatic prediction enable a high-confidence apicoplast proteome in malaria parasites. PLoS Biol. 2018 Sep 13;16(9):e2005895. PMID:
Comparison was made with list of nuclear genes with apicoplast signal sequences taken from PlasmoDB
Functional class Reference Annotation Pf Essentiality Present in gene list Transcript EC#
35048 DNA metabolism PF3D7_0103600
ATP-dependent DNA/RNA helicase psh1 Mutable in CDS NO
3.6.4.13
35194 PF3D7_1223300
DNA gyrase subunit A Non - Mutable in CDS YES
5.99.1.3
35200 PF3D7_1239500
DNA gyrase subunit B Non - Mutable in CDS YES
5.99.1.3
35211 PF3D7_1313400 DEAD box helicase, putative Mutable in CDS YES
3.6.1.3
35233 PF3D7_1405400 DNA mismatch repair protein, putative Mutable in CDS NO
35055 PF3D7_0204600 5'-3' exonuclease, putative Mutable in CDS NO
3.1.11.-
35170 PF3D7_1106300 5'-3' exoribonuclease 1, putative Non - Mutable in CDS YES
3.1.4.1
35091 PF3D7_0508800
single-stranded DNA-binding protein Non - Mutable in CDS YES
35206 PF3D7_1304100
DNA ligase I Non - Mutable in CDS YES
6.5.1.1
35237 PF3D7_1411400
plastid replication-repair enzyme Non - Mutable in CDS YES
2.7.7.7
35053 PF3D7_0112000 TatD-like deoxyribonuclease Non - Mutable in CDS YES
3.1.21.-
35087 PF3D7_0504400
ATP-dependent helicase, putative Non - Mutable in CDS YES
3.6.1.3
35207 PF3D7_1307600
DNA-directed RNA polymerase subunit alpha, putative Non - Mutable in CDS YES
2.7.7.6
35054 PF3D7_0203900
5'-3' exonuclease, putative Non - Mutable in CDS YES
3.1.16.1
35265 PF3D7_1472700 DNA-directed RNA polymerase, alpha subunit, putative Non - Mutable in CDS YES
2.7.7.6
35217 PF3D7_1332600
DNA-(apurinic or apyrimidinic site) lyase 1 Mutable in CDS YES
4.2.99.18
38907 RNA metabolism PF3D7_0319200
ccr4 domain-containing protein 4, putative Mutable in CDS YES
35076 PF3D7_0408900
tRNA N6-adenosine threonylcarbamoyltransferase Non - Mutable in CDS YES
2.6.99.4
35115 PF3D7_0707800 RAP protein, putative Non - Mutable in CDS NO
35120 PF3D7_0727500 mterf domain-containing protein, putative Non - Mutable in CDS NO
35202 PF3D7_1244000 glucose inhibited division protein a homologue, putative Non - Mutable in CDS YES
35203 PF3D7_1244400 RNA-binding protein, putative Non - Mutable in CDS NO
35144 PF3D7_0914300 met-10+ like protein, putative Mutable in CDS YES
2.1.1.-
35161 PF3D7_1018000 tRNA pseudouridine synthase, putative Mutable in CDS YES
5.4.99.12
35193 PF3D7_1221700 FbpA domain protein, putative Non - Mutable in CDS NO
35074 PF3D7_0405400 pre-mRNA-processing-splicing factor 8, putative Non - Mutable in CDS NO
35112 PF3D7_0627700 transportin Non - Mutable in CDS NO
35119 PF3D7_0723700 metallo-hydrolase/oxidoreductase, putative Non - Mutable in CDS NO
3.1.26.11
35094 PF3D7_0516300 tRNA pseudouridine synthase, putative Mutable in CDS NO
4.2.1.70
35225 PF3D7_1350700 N6-adenine-specific methylase, putative Non - Mutable in CDS YES
2.1.1.62
35096 PF3D7_0518100
protein amr2 Mutable in CDS NO
35125 PF3D7_0811900 RNA-binding protein, putative Non - Mutable in CDS NO
35174 PF3D7_1120500 tRNA nucleotidyltransferase, putative Non - Mutable in CDS YES
2.7.7.72
35086 PF3D7_0504200 ATP-dependent RNA helicase ddx27, putative Non - Mutable in CDS YES
3.6.4.13
35061 PF3D7_0218300 rRNA methyltransferase, putative Mutable in CDS YES
2.1.1.-
35062 PF3D7_0219500
pseudouridine synthase, putative Mutable in CDS YES
4.2.1.70
35201 PF3D7_1242200
queuine tRNA-ribosyltransferase, putative Non - Mutable in CDS YES
2.4.2.29
35131 PF3D7_0817100 tRNA modification GTPase, putative Non - Mutable in CDS NO
35199 PF3D7_1236700 S-adenosyl-methyltransferase, putative Non - Mutable in CDS YES
2.1.1.12
35147 PF3D7_0918800 dihydrouridine synthase, putative Mutable in CDS YES
1.3.1.42
35183 PF3D7_1207500 RNA-binding protein, putative Non - Mutable in CDS NO
35075 PF3D7_0407200 peptidyl-tRNA hydrolase 2, putative Non - Mutable in CDS YES
3.1.1.29
35143 PF3D7_0914000 pseudouridylate synthase, putative Mutable in CDS YES
4.2.1.70
35177 PF3D7_1133800
RNA (uracil-5-)methyltransferase, putative Non - Mutable in CDS NO
2.1.1.35
35149 PF3D7_0920200 CS domain protein, putative Non - Mutable in CDS NO
35058 Translation PF3D7_0208600
ribosome-recycling factor Non - Mutable in CDS YES
35079 PF3D7_0410700 ribosome biogenesis GTPase A, putative Non - Mutable in CDS YES
3.6.5.1
35081 PF3D7_0414700 GTP-binding protein, putative Mutable in CDS YES
35092 PF3D7_0509600
asparagine--tRNA ligase Non - Mutable in CDS YES
6.1.1.22
35093 PF3D7_0514300 aspartate--tRNA ligase, putative Non - Mutable in CDS YES
6.1.1.12
35103 PF3D7_0602400
elongation factor G Non - Mutable in CDS YES
35133 PF3D7_0827500 ribosomal protein l21, apicoplast, putative Non - Mutable in CDS YES
35134 PF3D7_0828200 leucine--tRNA ligase, putative Non - Mutable in CDS YES
6.1.1.4
35139 PF3D7_0911700 GTP-binding protein, putative Mutable in CDS YES
35142 PF3D7_0913900
arginine--tRNA ligase, putative Non - Mutable in CDS YES
6.1.1.19
35156 PF3D7_0934000 histidine--tRNA ligase, putative Mutable in CDS YES
6.1.1.21
35067 PF3D7_0311200 valine--tRNA ligase, putative Non - Mutable in CDS YES
6.1.1.9
35164 PF3D7_1032000 ribosome maturation factor RimM, putative Non - Mutable in CDS NO
35192 PF3D7_1217300
GTP-binding protein ENGA Non - Mutable in CDS YES
35195 PF3D7_1225100
isoleucine--tRNA ligase, putative Mutable in CDS YES
6.1.1.5
35196 PF3D7_1232000
phenylalanine--tRNA ligase Non - Mutable in CDS YES
6.1.1.20
35239 PF3D7_1413400 30S ribosomal protein S9, putative Non - Mutable in CDS YES
35064 PF3D7_0305000
elongation factor Ts Non - Mutable in CDS YES
35095 PF3D7_0516600
translation initiation factor IF-2 sporozoite surface antigen MB2 Non - Mutable in CDS YES
35099 PF3D7_0524600 50S ribosomal protein L12, apicoplast, putative Non - Mutable in CDS YES
35114 PF3D7_0704900 peptide chain release factor 2 Non - Mutable in CDS YES
3.1.1.29
35238 PF3D7_1411600
GTP-binding protein protein obg1 Non - Mutable in CDS YES
35245 PF3D7_1428600
peptide chain release factor 1 Non - Mutable in CDS YES 3.1.1.29
35246 PF3D7_1429100 ribosomal protein l15, apicoplast, putative Non - Mutable in CDS YES
35241 PF3D7_1416800
lysine--tRNA ligase, putative Non - Mutable in CDS YES
6.1.1.6
35137 PF3D7_0907900
peptide deformylase Non - Mutable in CDS YES
3.5.1.88
35158 PF3D7_1005000
methionine--tRNA ligase, putative Non - Mutable in CDS YES
6.1.1.10
35210 PF3D7_1313200
methionyl-tRNA formyltransferase, putative Mutable in CDS YES
2.1.2.9
35153 PF3D7_0925300
proline--tRNA ligase, putative Non - Mutable in CDS YES
6.1.1.15
35231 PF3D7_1367700
alanine--tRNA ligase Non - Mutable in CDS YES
6.1.1.7
35146 PF3D7_0918200 50S ribosomal protein L3, apicoplast, putative Non - Mutable in CDS YES
35244 PF3D7_1420400
glycine--tRNA ligase Non - Mutable in CDS YES
6.1.1.14
35172 PF3D7_1117500
tyrosine--tRNA ligase Non - Mutable in CDS YES
6.1.1.1
35191 PF3D7_1216000 serine--tRNA ligase, putative Non - Mutable in CDS YES
6.1.1.11
35176 PF3D7_1126000
threonine--tRNA ligase Non - Mutable in CDS YES
6.1.1.3
35205 PF3D7_1251700
tryptophanyl-tRNA synthetase, putative tryptophan--tRNA ligase, putative Non - Mutable in CDS YES 6.1.1.2
35160 PF3D7_1015200
cysteine--tRNA ligase, putative Non - Mutable in CDS YES
6.1.1.16
35260 PF3D7_1460900 ribosomal protein S10, apicoplast, putative Non - Mutable in CDS YES
35260 PF3D7_1460900 ribosomal protein S10, apicoplast, putative Non - Mutable in CDS YES
35169 PF3D7_1106100 ribosomal protein s15, apicoplast, putative Non - Mutable in CDS YES
35155 PF3D7_0932600 apicoplast ribosomal protein S6, putative Mutable in CDS YES
35157 PF3D7_1003200 ribosome-binding factor A, putative Non - Mutable in CDS NO
35204 PF3D7_1249900 ribosomal RNA small subunit methyltransferase a2, putative Non - Mutable in CDS YES
35078 PF3D7_0409700 peptide chain release factor 2, putative Non - Mutable in CDS NO
3.1.1.29
35185 PF3D7_1208000 ribosomal protein L29, apicoplast, putative Non - Mutable in CDS YES
35165 PF3D7_1034200 ribosomal protein l27, putative Non - Mutable in CDS YES
35124 PF3D7_0810100 ribosomal protein l33, apicoplast, putative Non - Mutable in CDS NO
35066 PF3D7_0310000 50S ribosomal protein L9, apicoplast, putative Non - Mutable in CDS NO
35097 PF3D7_0522500 50S ribosomal protein L17, apicoplast, putative Non - Mutable in CDS YES
35080 PF3D7_0413800 50S ribosomal protein L10, putative Non - Mutable in CDS YES
35188 PF3D7_1210000 50S ribosomal protein L1, apicoplast, putative Non - Mutable in CDS YES
35136 PF3D7_0907800 ribosomal protein L35, apicoplast, putative Non - Mutable in CDS YES
35106 PF3D7_0613400 50s ribosomal protein l18, apicoplast, putative Non - Mutable in CDS YES
35262 PF3D7_1467400
50S ribosomal protein L22, apicoplast, putative Non - Mutable in CDS YES
35179 PF3D7_1137500
ribosomal protein s14, apicoplast, putative Non - Mutable in CDS YES
35226 PF3D7_1357200
glutamate--tRNA ligase Non - Mutable in CDS YES
6.1.1.17
35263 PF3D7_1469000
translation initiation factor IF-1 Non - Mutable in CDS YES
35166 PF3D7_1034600
translation initiation factor IF-3, putative Non - Mutable in CDS NO
35116 PF3D7_0711000
AAA family ATPase, CDC48 subfamily Non - Mutable in CDS YES
3.6.4.6
35180 PF3D7_1144700
apicoplast import protein Tic20, putative Non - Mutable in CDS NO
35198 PF3D7_1234600 protein TOC75, putative Non - Mutable in CDS NO
35228 Post-translational modifications PF3D7_1358300
rhomboid protease ROM7 Mutable in CDS NO
3.4.21.105
35234 PF3D7_1406400
pentatricopeptide repeat domain-containing protein 1 Non - Mutable in CDS NO
35126 PF3D7_0812220
GTP-binding protein YihA3 Non - Mutable in CDS NO
35128 PF3D7_0815700
apicoplast ubiquitin-like protein aubl Non - Mutable in CDS YES
35130 PF3D7_0816600
chaperone protein ClpB1 Non - Mutable in CDS YES
35251 PF3D7_1440200
stromal-processing peptidase, putative Non - Mutable in CDS YES
3.4.24.56
35208 PF3D7_1308800 tyrosine recombinase Non - Mutable in CDS NO
35197 PF3D7_1232100
60 kDa chaperonin Non - Mutable in CDS YES
35254 PF3D7_1446200
M17 leucyl aminopeptidase Non - Mutable in CDS YES
3.4.11.1
35218 PF3D7_1333000
20 kDa chaperonin Non - Mutable in CDS YES
35253 PF3D7_1443900
heat shock protein 90, putative Non - Mutable in CDS YES
35219 PF3D7_1333200
ubiquitin-activating enzyme Non - Mutable in CDS YES
6.2.1.45
35235 PF3D7_1406600
ATP-dependent Clp protease regulatory subunit Clpc, putative Non - Mutable in CDS YES
3.4.21.92
35221 PF3D7_1338900 serine/threonine protein kinase, putative Non - Mutable in CDS YES
2.7.11.1
35141 PF3D7_0913500
apicoplast-minus ipp-rescued 4 Non - Mutable in CDS YES
35140 PF3D7_0912400
alkaline phosphatase, putative Mutable in CDS NO
3.1.3.1
35159 PF3D7_1009600 protein phosphatase ppm10, putative Non - Mutable in CDS NO
3.1.3.16
35088 PF3D7_0507200
subtilisin-like protease 3 Mutable in CDS NO
3.4.21.62
35065 PF3D7_0307400
ATP-dependent Clp protease proteolytic subunit Non - Mutable in CDS YES
3.4.21.92
35248 PF3D7_1436800 ATP-dependent Clp protease proteolytic subunit Non - Mutable in CDS YES
3.4.21.92
35077 PF3D7_0409400