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Essential metabolic genes |
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Carey MA, Papin JA, Guler JL. Novel Plasmodium falciparum metabolic network reconstruction identifies shifts associated with clinical antimalarial resistance. BMC Genomics. 2017 18(1):543. PMID: 28724354 |
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PfID |
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Enzyme |
EC |
In vitro method |
Transcript |
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| 40840 |
PF3D7_1420600 |
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pantothenate kinase 1 |
2.7.1.33 |
Inhibitors (several) |
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| 40842 |
PF3D7_1437400 |
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pantothenate kinase 2 |
2.7.1.33 |
Inhibitors (several) |
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| 40795 |
PF3D7_0810800 |
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hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase |
2.5.1.15 |
Inhibitors (several) |
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| 40786 |
PF3D7_0417200 |
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bifunctional dihydrofolate reductase-thymidylate synthase |
1.5.1.3 |
Inhibitor (1843U89) |
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| 40824 |
PF3D7_1324900 |
L-lactate dehydrogenase |
1.1.1.27 |
Inhibitors (Gossypol, others) |
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| 40825 |
PF3D7_1325200 |
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lactate dehydrogenase |
1.1.1.27 |
Inhibitors (Gossypol, others) |
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| 40847 |
PF3D7_1467300 |
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1-deoxy-D-xylulose 5-phosphate reductoisomerase |
1.1.1.267 |
Inhibitor (Fosmidomycin) |
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| 40807 |
PF3D7_1033100 |
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S-adenosylmethionine decarboxylase/ornithine decarboxylase |
4.1.1.17 |
Inhibitors (3-Aminooxy-1-aminopropane, DFMO) |
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| 40813 |
PF3D7_1129000 |
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spermidine synthase |
2.5.1.16 |
Inhibitors (4MCHA and others) |
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| 40792 |
PF3D7_0615100 |
enoyl-acyl carrier reductase |
1.3.1.9 |
Inhibitor (Triclosan)* |
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| 40794 |
PF3D7_0626300 |
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3-oxoacyl-acyl-carrier protein synthase I/II |
2.3.1.41 |
Inhibitor (Cerulenin) |
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| 40801 |
PF3D7_1012400 |
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hypoxanthine-guanine phosphoribosyltransferase |
2.4.2.8 |
Antisense oligos |
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| 40833 |
PF3D7_1354500 |
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adenylosuccinate synthetase |
6.3.4.4 |
Inhibitor (hadacidin) |
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| 40784 |
PF3D7_0206700 |
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adenylosuccinate lyase |
4.3.2.2 |
Inhibitors (5-aminoimidazole-4-carboxamide ribonucleoside) |
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| 40788 |
PF3D7_0603300 |
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dihydroorotate dehydrogenase |
1.3.5.2 |
RNAi; Inhibitors (several) |
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| 40806 |
PF3D7_1029600 |
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adenosine deaminase |
3.5.4.4 |
Inhibitor (methylthiocoformycin) |
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| 40787 |
PF3D7_0513300 |
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purine nucleoside phosphorylase |
2.4.2.1 |
Inhibitor (immucillin-H) |
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| 40802 |
PF3D7_1015800 |
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ribonucleoside-diphosphate reductase small chain, putative |
1.17.4.1 |
Oligodeoxynucleotide phosphorothioate |
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| 40835 |
PF3D7_1405600 |
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ribonucleoside-diphosphate reductase small chain, putative |
1.17.4.1 |
Oligodeoxynucleotide phosphorothioate |
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| 40841 |
PF3D7_1437200 |
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ribonucleoside-diphosphate reductase large subunit, putative |
1.17.4.1 |
Oligodeoxynucleotide phosphorothioate |
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| 40892 |
PF3D7_1140000 |
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carbonic anhydrase |
4.2.1.1 |
Inhibitors (several) |
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| 40823 |
PF3D7_1308200 |
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carbamoyl phosphate synthetase |
6.3.5.5 |
Ribozymes |
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| 40811 |
PF3D7_1127100 |
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deoxyuridine 5'-triphosphate nucleotidohydrolase dUTP pyrophosphatase |
3.6.1.23 |
Inhibitors (several) |
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| 40810 |
PF3D7_1113700 |
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glyoxalase I |
4.4.1.5 |
Inhibitor (S-p-bromobenzylglutathione diethyl ester) |
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| 40789 |
PF3D7_0604700 |
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glyoxalase I-like protein gilp |
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Inhibitor (S-p-bromobenzylglutathione diethyl ester) |
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| 40800 |
PF3D7_0923800 |
thioredoxin reductase |
1.8.1.9 |
Gene KO |
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| 40849 |
PF3D7_0206400 |
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pentafunctional AROM polypeptide, putative, pseudogene |
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Inhibitor (glyphosate) |
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| 40793 |
PF3D7_0623000 |
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chorismate synthase |
4.2.3.5 |
RNAi |
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| 40818 |
PF3D7_1238600 |
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sphingomyelin phosphodiesterase |
3.1.4.12 |
Inhibitor (Scyphostatin) |
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| 40836 |
PF3D7_1408000 |
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plasmepsin II |
3.4.23.39 |
Inhibitors (several) |
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| 40817 |
PF3D7_1225100 |
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isoleucine--tRNA ligase, putative |
6.1.1.5 |
Inhibitor (mupirocin) |
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| 40827 |
PF3D7_1332900 |
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isoleucine--tRNA ligase, putative |
6.1.1.5 |
Inhibitor (Thiaisoleucine) |
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| 40797 |
PF3D7_0918900 |
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gamma-glutamylcysteine synthetase |
6.3.2.2 |
Inhibitor (L-buthionine sulfoximine); fail to Gene KO |
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| 40838 |
PF3D7_1419300 |
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glutathione S-transferase |
2.5.1.18 |
Inhibitors (ellagic acid, others) |
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| 40839 |
PF3D7_1419800 |
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glutathione reductase |
1.8.1.7 |
Inhibitors (several) fail to KO |
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| 40812 |
PF3D7_1128400 |
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geranylgeranyl pyrophosphate synthase, putative |
2.5.1.29 |
Inhibitors (many) |
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| 40812 |
PF3D7_1128400 |
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geranylgeranyl pyrophosphate synthase, putative |
2.5.1.29 |
Inhibitors (many) |
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| 40832 |
PF3D7_1351600 |
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glycerol kinase |
2.7.1.30 |
Gene KO |
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| 40844 |
PF3D7_1444800 |
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fructose-bisphosphate aldolase |
4.1.2.13 |
Antisense oligos |
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| 40820 |
PF3D7_1246100 |
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delta-aminolevulinic acid synthetase |
2.3.1.37 |
Gene KO |
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| 40834 |
PF3D7_1364900 |
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ferrochelatase, HemH |
4.99.1.1 |
Gene KO |
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| 40829 |
PF3D7_1342100 |
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aconitate hydratase |
4.2.1.3 |
Gene KO ; Inhibitor (Sodium fluoroacetate) |
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| 40837 |
PF3D7_1416500 |
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NADP-specific glutamate dehydrogenase |
1.4.1.4 |
Gene KO |
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| 40796 |
PF3D7_0820700 |
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2-oxoglutarate dehydrogenase E1 component |
1.2.4.2 |
Gene KO |
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| 40809 |
PF3D7_1108500 |
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succinate--CoA ligase [ADP-forming] subunit alpha, putative |
6.2.1.4 |
Gene KO |
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| 40808 |
PF3D7_1034400 |
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flavoprotein subunit of succinate dehydrogenase |
1.3.5.1 |
Gene KO |
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| 40803 |
PF3D7_1022500 |
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citrate synthase, mitochondrial, putative |
2.3.3.1 |
Gene KO |
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| 40831 |
PF3D7_1345700 |
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isocitrate dehydrogenase [NADP], mitochondrial |
1.1.1.42 |
Gene KO |
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| 40805 |
PF3D7_1028100 |
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protoporphyrinogen oxidase |
1.3.3.4 |
Inhibitor (Acifluorfen) |
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| 40804 |
PF3D7_1026900 |
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biotin-protein ligase 1 |
6.4.1.2 |
Inhibitor (Haloxyfops, Fluazifops, Quizalofops) |
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| 40848 |
PF3D7_1469600 |
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acetyl-coa carboxylase |
6.3.4.14 |
Inhibitor (Haloxyfops, Fluazifops, Quizalofops) |
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| 40843 |
PF3D7_1440300 |
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delta-aminolevulinic acid dehydratase porphobilinogen synthase, HemB |
4.2.1.24 |
Inhibitor (Succinyl acetone) |
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Bold = conflicting experimental results |
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Additional essential metabolic genes |
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Oyelade J, Isewon I, Uwoghiren E, Aromolaran O, Oladipupo O. In Silico Knockout Screening of Plasmodium falciparum Reactions and Prediction of Novel Essential Reactions by Analysing the Metabolic Network. Biomed Res Int. 2018 29;2018:8985718. PMID:2978980 |
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| 40814 |
PF3D7_1142400 |
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coproporphyrinogen-III oxidase HemF |
1.3.3.3 |
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| 40846 |
PF3D7_1450900 |
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acetyl-CoA acetyltransferase, putative |
2.3.1.9 |
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| 40798 |
PF3D7_0922200 |
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S-adenosylmethionine synthetase |
2.5.1.6 |
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| 40815 |
PF3D7_1209600 |
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porphobilinogen deaminase |
2.5.1.61 |
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| 40791 |
PF3D7_0608800 |
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ornithine aminotransferase |
2.6.1.13 |
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| 40799 |
PF3D7_0922400 |
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para-aminobenzoic acid synthetase |
2.6.1.85 |
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| 40821 |
PF3D7_1251300 |
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thymidylate kinase |
2.7.4.9 |
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| 40826 |
PF3D7_1327600 |
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nicotinamide/nicotinic acid mononucleotide adenylyltransferase |
2.7.7.18 |
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| 40830 |
PF3D7_1343600 |
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UDP-N-acetylglucosamine pyrophosphorylase, putative |
2.7.7.23 |
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| 40785 |
PF3D7_0321200 |
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UDP-N-acetylglucosamine--dolichyl-phosphate n-acetylglucosaminephosphotransferase, putative |
2.7.8.15 |
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| 40819 |
PF3D7_1240000 |
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3-hydroxyisobutyryl-CoA hydrolase, putative |
4.2.1.17 |
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| 40816 |
PF3D7_1224000 |
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GTP cyclohydrolase I |
3.5.4.16 |
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| 40790 |
PF3D7_0607300 |
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uroporphyrinogen III decarboxylase |
4.1.1.37 |
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| 40828 |
PF3D7_1336900 |
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tryptophanyl-tRNA synthetase tryptophan--tRNA ligase |
6.1.1.2 |
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| 40822 |
PF3D7_1251700 |
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tryptophanyl-tRNA synthetase, putative tryptophan--tRNA ligase, putative |
6.1.1.2 |
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| 40845 |
PF3D7_1445100 |
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histidine--tRNA ligase, putative |
6.1.1.21 |
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