Gametocyte proteomics
Lasonder E, Rijpma SR, van Schaijk BC, Hoeijmakers WA, Kensche PR, Gresnigt MS, Italiaander A, Vos MW, Woestenenk R, Bousema T, Mair GR, Khan SM, Janse CJ, Bártfai R, Sauerwein RW. Integrated transcriptomic and proteomic analyses of P. falciparum translational repression. Nucleic Acids Res. 2016 Jul 27;44(13):6087-101. PMID: 27298255;
LFQ = label-free protein quantification; FG = female gametocyte; MG = Male gametocyte
known marker-proteins of Plasmodium female (FG) or male (MG) gametocytes
PfID Annotation RATIO LFQ FG/MG Differentially Expressed Protein Sex EC Transcript
42876 PF3D7_0109100
LCCL domain-containing protein 7805.09 +
43082 PF3D7_1250100
osmiophilic body protein g377 5874.60 + FG
43150 PF3D7_1455800
LCCL domain-containing protein 4112.94 +
43148 PF3D7_1451600
LCCL domain-containing protein 3504.15 +
43102 PF3D7_1346800
6-cysteine protein p47 2211.25 +
42924 PF3D7_0513700 secreted ookinete protein, putative 6-cysteine protein 2161.65 +
43166 PF3D7_1475500
LCCL domain-containing protein 2087.32 + FG
43138 PF3D7_1426500
ABC transporter G family member 2 1450.09 + FG
43134 PF3D7_1420900
mannose-1-phosphate guanyltransferase, putative 1341.55 + 2.7.7.13
43118 PF3D7_1407000
LCCL domain-containing protein 809.16 + FG
42874 PF3D7_0103800 actin-related protein arp1, putative 524.66 +
42911 PF3D7_0417000 conserved Plasmodium protein, unknown function 346.86 +
43042 PF3D7_1128300 ATP-dependent 6-phosphofructokinase 329.03 + 2.7.1.11
43159 PF3D7_1466800 noc3 domain-containing protein, putative 311.50 +
42960 PF3D7_0723200 conserved Plasmodium protein, unknown function 276.79
43011 PF3D7_1021900 PHAX domain-containing protein 201.25 +
42953 PF3D7_0704400 phosphoinositide-binding protein, putative 197.58
43010 PF3D7_1017400 phosphomannomutase 190.37 5.4.2.8
43110 PF3D7_1367600 ribosome biogenesis protein MRT4, putative 181.94 +
43162 PF3D7_1472900
dihydroorotase, putative 169.41 + 3.5.2.3
43062 PF3D7_1218800 secreted ookinete protein, putative 161.22 +
42895 PF3D7_0314000 hsp20-like chaperone, putative 159.50 +
43034 PF3D7_1116900 conserved Plasmodium protein, unknown function 157.94 +
42967 PF3D7_0806200 dpy-19-like c-mannosyltransferase 140.40 + 2.4.1.-
42922 PF3D7_0513000 conserved Plasmodium protein, unknown function 130.70 +
43040 PF3D7_1123300 RING zinc finger protein, putative 130.20
42882 PF3D7_0207600
serine repeat antigen 5 125.96 3.4.22.-
43031 PF3D7_1113300 UDP-galactose transporter, putative, UGT 120.28 +
43009 PF3D7_1016900
early transcribed membrane protein 10.3, UIS4 119.08
42978 PF3D7_0825900 conserved Plasmodium protein, unknown function 111.90 +
43125 PF3D7_1414500
atypical protein kinase, ABC-1 family, putative 111.78 + 1.14.13.-
43005 PF3D7_1012500 phosphoglucomutase, putative 110.90 + 5.4.2.2
42904 PF3D7_0323800 conserved Plasmodium protein, unknown function 107.21 +
43113 PF3D7_1401800
choline kinase 106.98 + 2.7.1.32
42959 PF3D7_0719200
NIMA related kinase 4 97.18 + FG 2.7.11.1
43165 PF3D7_1474900 trailer hitch homolog, putative 96.50 + FG
43028 PF3D7_1110700 actin-like protein, putative 96.26 +
42996 PF3D7_0922900
3-oxoacyl-[acyl-carrier-protein] reductase 91.44 + 1.1.1.35
43055 PF3D7_1206100
imp-specific 5'-nucleotidase 1 87.93 + 3.1.3.5
42894 PF3D7_0310300 phosphoglycerate mutase, putative 87.48 + 5.4.2.11
42984 PF3D7_0905400
high molecular weight rhoptry protein 3 85.86
43022 PF3D7_1034100 conserved Plasmodium protein, unknown function 74.15
42995 PF3D7_0921900 conserved Plasmodium protein, unknown function 70.15 +
43149 PF3D7_1451800
sortilin 68.98 +
43021 PF3D7_1033200
early transcribed membrane protein 10.2 68.22
42891 PF3D7_0306400 FAD-dependent glycerol-3-phosphate dehydrogenase, putative 68.03 + 1.1.5.3
42963 PF3D7_0730200 AP-4 complex subunit beta, putative 66.68
42947 PF3D7_0621100 conserved Plasmodium protein, unknown function 66.55 +
43140 PF3D7_1435300
glutamate synthase [NADH], putative 64.82 + 1.4.1.14
42875 PF3D7_0105200
RAP01 60.75 +
43047 PF3D7_1136300
tudor staphylococcal nuclease 59.01 + 3.1.31.1
42945 PF3D7_0618600 rhomboid protease ROM10 58.95 3.4.21.105
43091 PF3D7_1323500
PEXEL protease plasmepsin V 56.09 + 3.4.23.-
43144 PF3D7_1446500
Nup313 54.85
42896 PF3D7_0314200 conserved Plasmodium protein, unknown function 54.81
43064 PF3D7_1220000 zinc finger protein, putative 54.42 +
42902 PF3D7_0320800
ATP-dependent RNA helicase DDX6 52.58 + FG 3.6.4.13
42943 PF3D7_0608800
ornithine aminotransferase 52.38 + 2.6.1.13
42974 PF3D7_0819900 U6 snRNA-associated Sm-like protein LSm3, putative 48.72
43083 PF3D7_1251000 Hsp20-like chaperone, putative 45.91 +
42968 PF3D7_0808200
plasmepsin X 45.38 + 3.4.23.1
42897 PF3D7_0314700
ring finger protein rnf1 44.34 +
43143 PF3D7_1442900
sec7 domain-containing protein arfgef, putative 44.22 +
43006 PF3D7_1014000
GDP-L-fucose synthase 44.11 + 1.1.1.271
43015 PF3D7_1024800
exported protein 3 44.10 +
43106 PF3D7_1349600 conserved Plasmodium protein, unknown function 43.64 +
42927 PF3D7_0515600 gamete egress protein gep 42.34 +
43136 PF3D7_1425700 vacuolar er assembly factor vma12, putative 42.21 +
42940 PF3D7_0606800 VFT protein 41.58 +
43115 PF3D7_1403700
translocation associated membrane protein, putative 40.00 2.3.1.24
43070 PF3D7_1232100
60 kDa chaperonin 39.29 +
43131 PF3D7_1419800
glutathione reductase 38.80 + 1.8.1.7
42906 PF3D7_0409000 conserved Plasmodium protein, unknown function 38.05 +
42965 PF3D7_0802000
glutamate dehydrogenase, putative 37.74 + 1.4.1.2
42908 PF3D7_0411100 mediator of RNA polymerase II transcription subunit 8, 37.56
42972 PF3D7_0816600
chaperone protein ClpB1 36.14 +
43164 PF3D7_1474000 probable protein, unknown function 36.05 +
43032 PF3D7_1113400 ubiquitin domain-containing protein DSK2, putative 35.75 +
42936 PF3D7_0528900 conserved Plasmodium protein, unknown function 35.07
43155 PF3D7_1460400
ubiquitin carboxyl-terminal hydrolase isozyme L3 34.45 3.4.19.12
43141 PF3D7_1435700 ataxin-2 like protein, putative 34.39 +
42916 PF3D7_0501200
parasite-infected erythrocyte surface protein 34.11
43107 PF3D7_1356600 regulator of chromosome condensation, putative 33.54 +
43128 PF3D7_1416400 conserved Plasmodium protein, unknown function 33.41
43035 PF3D7_1118200 heat shock protein 90, putative 33.34 +
42900 PF3D7_0317700
CPSF (cleavage and polyadenylation specific factor), subunit A, putative 32.75
43075 PF3D7_1236900 conserved Plasmodium protein, unknown function 32.64 +
42987 PF3D7_0911200 conserved Plasmodium protein, unknown function 32.18
42990 PF3D7_0914500 conserved Plasmodium protein, unknown function 31.79 +
42964 PF3D7_0731600 acyl-CoA synthetase 31.60 6.2.1.3
43018 PF3D7_1027300
peroxiredoxin merozoite capping protein 1 31.54 + 1.11.1.15
43096 PF3D7_1333800 swelling-induced anion-selective channel 29.90
43023 PF3D7_1037100
pyruvate kinase 2, pyrkii 27.89 + 2.7.1.40
43036 PF3D7_1118900 conserved Plasmodium protein, unknown function 27.69 +
42986 PF3D7_0906400 dynein intermediate light chain, putative 25.83 +
42898 PF3D7_0316300 inorganic pyrophosphatase 24.93 + 3.6.1.1
43120 PF3D7_1409800 CUGBP Elav-like family member 2 24.88 +
42928 PF3D7_0516400 phosducin-like protein 1, putative 24.34
43029 PF3D7_1111900 Josephin domain-containing protein, putative 23.78 + 3.4.19.12
43097 PF3D7_1342800
phosphoenolpyruvate carboxykinase 23.36 + 4.1.1.49
42881 PF3D7_0206000 DNA repair protein RAD2, putative 23.13 4.2.99.18
43085 PF3D7_1302000
EMP1-trafficking protein 22.93
42962 PF3D7_0729900
dynein heavy chain, putative 22.56 + FG
42955 PF3D7_0708300
EKC/KEOPS complex subunit BUD32 21.72 + 2.7.11.2
43080 PF3D7_1247800
dipeptidyl aminopeptidase 2 21.68 + 3.4.14.1
43056 PF3D7_1209200 U6 snRNA-associated Sm-like protein LSm7, putative 21.21
42931 PF3D7_0517600 F-actin-capping protein subunit beta, putative 21.08 +
43063 PF3D7_1219100
clathrin heavy chain, putative 20.44 +
43090 PF3D7_1320800
dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 20.38 + 2.3.1.61
43112 PF3D7_1372100 Plasmodium exported protein (PHISTb), unknown function gametocyte exported protein 4 20.28
43130 PF3D7_1419200 thioredoxin-like protein, putative 20.27
43153 PF3D7_1457300 conserved Plasmodium protein, unknown function 20.11 +
43048 PF3D7_1139500
AAA family ATPase, putative 19.67 + 3.4.21.92
42887 PF3D7_0303000
N-ethylmaleimide-sensitive fusion protein 19.65 + 3.6.4.6
43109 PF3D7_1361800 glideosome-associated connector 19.19 +
42925 PF3D7_0514600 ribose-5-phosphate isomerase 18.85 5.3.1.6
43014 PF3D7_1024300 ATP synthase-associated protein, ATPTG10 18.67 +
43094 PF3D7_1330000
pith domain-containing protein, putative 17.88 +
43129 PF3D7_1417900 cleavage and polyadenylation specificity factor subunit 4, putative 17.86
43099 PF3D7_1343800 vps13 domain-containing protein, putative 17.73 +
43081 PF3D7_1248600 conserved Plasmodium protein, unknown function 17.26 +
42971 PF3D7_0811600 conserved Plasmodium protein, unknown function 17.21 +
43147 PF3D7_1450300
NADPH--cytochrome P450 reductase, putative 17.03
43084 PF3D7_1253000
gametocyte exported protein 1 gametocyte erythrocyte cytosolic protein 16.90 +
42951 PF3D7_0626700 ATPase, putative 16.51 +
43105 PF3D7_1347700
ethanolamine-phosphate cytidylyltransferase 16.36 + 2.7.7.14
42920 PF3D7_0508200
longevity-assurance (LAG1) protein, putative 15.52 + 2.3.1.24
42929 PF3D7_0517300
serine/arginine-rich splicing factor 1 15.49
43101 PF3D7_1346000 dynactin subunit 2, putative 15.48
42998 PF3D7_0928000 COX6b cytochrome c oxidase subunit 6B, putative 15.44 1.9.3.1
43001 PF3D7_1002400 transformer-2 protein homolog beta, putative 15.08 +
43045 PF3D7_1134000
heat shock protein 70 15.06 +
42981 PF3D7_0828900 conserved Plasmodium protein, unknown function 15.00 +
43111 PF3D7_1369400 conserved Plasmodium protein, unknown function 14.92
42932 PF3D7_0519500 ccr4 domain-containing protein 1, putative 14.83
43050 PF3D7_1145400
dynamin-like protein 14.77 +
42980 PF3D7_0828800
GPI-anchored micronemal antigen 14.74 +
42884 PF3D7_0215000 acyl-CoA synthetase 14.59 + 6.2.1.3
42903 PF3D7_0322400 regulator of initiation factor 2 (eIF2) 14.50
43121 PF3D7_1410700 conserved Plasmodium protein, unknown function 14.40
42969 PF3D7_0810000 acyl-CoA binding protein, putative 14.37
42957 PF3D7_0716800 eukaryotic translation initiation factor 3 subunit I, putative 14.05 +
43012 PF3D7_1022500
citrate synthase, mitochondrial, putative 13.95 + 2.3.3.1
42939 PF3D7_0605900 elongation of fatty acids protein, putative 13.83 + 6.2.1.3
42892 PF3D7_0306500 Cytochrome c oxidase subunit 6C, putative 13.77 +
42976 PF3D7_0821000 conserved Plasmodium protein, unknown function 13.75
42988 PF3D7_0912900 26S proteasome regulatory subunit RPN8, putative 13.72 +
42905 PF3D7_0402300
reticulocyte binding protein homologue 1 normocyte binding protein 1 13.60
43041 PF3D7_1123400 eukaryotic peptide chain release factor GTP-binding subunit, putative, erf3 13.56 +
43093 PF3D7_1329100
myosin F 13.42 +
42917 PF3D7_0502400
ring-stage membrane protein 1 merozoite surface protein 8 13.42