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Protein factors in pre-mRNA 3’-end processing |
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Mammalian factor (yeast factor) |
Mammalian protein (yeast homolog) |
PfID |
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Annotation |
Transcript |
Mammalian protein partners (yeast protein partners) |
Protein function and characteristics |
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| 1125 |
CPSF (CPF) |
CPSF-160 |
PF3D7_0317700 |
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CPSF (cleavage and polyadenylation specific factor), subunit A, putative |
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CstF-77, Pol II CTD, PAP, hFip1, TFIID |
Binds RNA (mammalian AAUAAA site, yeast cleavage site), three possible b-propellers |
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(Cft1p/Yhh1p) |
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(Fip1p, Pol II CTD, Cft2p/Ydh1p and Swd2p) |
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| 1127 |
CPSF (CPF) |
CPSF-100 |
PF3D7_0318600 |
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cleavage and polyadenylation specificity factor, putative |
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CPSF-73 |
Binds RNA at the U-rich sequence in yeast, may act as a scaffolding protein, contains a non-metal binding metallo-blactamase domain |
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(Cft2p/Ydh1p) |
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(Pol II CTD, Brr5p/Ysh1p, Cft1p/Yhh1p, Pta1p, Pfs2p, Ssu72p, Pcf11p, YDL094C) |
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| 1141 |
CPSF (CPF) |
CPSF-73 |
PF3D7_1438500 |
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cleavage and polyadenylation specificity factor subunit 3, putative |
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CPSF-100 |
Endonuclease for the cleavage reaction, contains a metal binding metallo-b-lactamase domain |
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(Brr5p/Ysh1p) |
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(Pta1p, Cft2p/Ydh1p, Yth1p, Clp1p, Syc1p, Swd2p) |
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| 23980 |
CPSF (CPF) |
CPSF-30 |
PF3D7_1008600 |
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zinc finger (ccch type) protein, putative |
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hFip1 |
Binds U-richRNAsequences, contains five zinc fingers and a zinc knuckle (mammalian) |
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(Yth1p) |
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(Fip1p, Brr5p/Ysh1p) |
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| 1139 |
CPSF (CPF) |
hFip1 |
PF3D7_1321700 |
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splicing factor 1 |
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PAP, CPSF-160, CPSF-30, CstF-77 |
Directs PAP to correct polyadenylation site and binds U-rich sequences, contains a conserved region that binds CPSF-30 |
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(Fip1p) |
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(Pap1p, Yth1p, Rna14p) |
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| 1129 |
CstF (CF IA) |
CstF-77 |
PF3D7_0526500 |
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conserved Plasmodium protein, unknown function |
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CPSF-160, CstF64, CstF50, |
Scaffolding protein, links CstF and CPSF, contains a HAT domain and a Pro-rich segment |
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(Rna14p) |
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Pol II CTD (Rna15p, Pcf11p) |
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| 29794 |
CstF (CF IA) |
CstF-64 |
PF3D7_1224300 |
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polyadenylate-binding protein 1, putative |
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CstF-77, Symplekin |
Binds to RNA through an N-terminal RRM (G/U rich sequence in mammals, A-rich sequence in yeast) |
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| 1136 |
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PF3D7_1107300 |
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polyadenylate-binding protein-interacting protein 1, putative |
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| 25745 |
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(Rna15p) |
PF3D7_0933000 |
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cstf domain-containing protein, putative |
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(Pcf11p, Rna14p) |
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| 1130 |
CstF |
CstF-50 |
PF3D7_0620500 |
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cleavage stimulation factor subunit 1, putative |
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CstF-77, Pol II CTD, BARD1 |
Binds Pol II CTD, contains seven WD-40 repeats |
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| 1124 |
CF Im |
CF Im-25kD |
PF3D7_0109200 |
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cleavage and polyadenylation specificity factor subunit 5 |
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PAP, CF Im-68kD |
CF Im aids AAUAAA site recognition, contains a conserved PAP interaction domain |
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| 1128 |
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(None) |
PF3D7_0403700 |
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pre-mRNA-splicing factor CLF1, putative |
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| 1140 |
CF Im |
CF Im-68kD |
PF3D7_1420000 |
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splicing factor 3B subunit 4, putative |
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CF Im-25kD |
CF Im aids AAUAAA site recognition, contains an N-terminal RBD |
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(None) |
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| 1124 |
CF Iim (CF IA) |
hPcf11 |
PF3D7_0109200 |
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cleavage and polyadenylation specificity factor subunit 5 |
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(Pol II CTD, Rna14p, Rna15p, Clp1p, Swd2p, Cft1p/Yhh1p, Cft2p/Ydh1p, Brr5p/Ysh1p, Pta1p) |
Interacts with Pol II CTD, acts as a scaffolding protein, contains two zinc finger domains |
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(Pcf11p) |
no |
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CF Iim (CF IA) |
hClp1 |
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CF Im and CPSF |
TethersCPSFwithCFIm, contains Walker A and B motifs for ATP binding |
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| 23877 |
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(Clp1p) |
PF3D7_0821600 |
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polyribonucleotide 5'-hydroxyl-kinase clp1, putative |
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(Brr5p/Ysh1p, Cft2p/Ydh1p, Pcf11p) |
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– (CPF) |
Symplekin |
No |
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CstF-64, CPSF |
Bridges interactions between CPSF and CstF, regulated by phosphorylation |
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(Pta1p) |
No |
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(Brr5p/Ysh1p, Syc1p, Cft2p/Ydh1p, Swd2p, Glc7p, Pti1p, Ssu72p) |
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| 1138 |
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PAP |
PF3D7_1232900 |
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nucleotidyltransferase, putative |
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hFip1, CF Im, CPSF-160 (Fip1p) |
Adds poly(A) tail to cleaved pre-mRNA |
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| 1131 |
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(Pap1p) |
PF3D7_0625600 |
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poly(A) polymerase PAP, putative |
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| 1126 |
– |
Pol II CTD |
PF3D7_0318200 |
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DNA-directed RNA polymerase II subunit RPB1 |
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hPcf11, CPSF-160, CstF-50, CstF-77 |
Transcriptional termination, contains YSPTSPS repeats |
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(Pol II CTD) |
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(Cft2p/Ydh1p, Cft1p/Yhh1p, Pcf11p) |
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| 29881 |
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PABII |
PF3D7_0923900 |
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polyadenylate-binding protein 2, putative |
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(Rna15p) |
Stimulates polyadenylation and stabilizes poly(A) tail, contains two RRM domains |
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(Pab1p) |
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Notice that Pf are generally orthologous to mammalian genes rather than to yeast |
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