|
Prenylated proteins |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Suazo KF, Schaber C, Palsuledesai CC, Odom John AR, Distefano MD. Global proteomic analysis of prenylated proteins in Plasmodium falciparum using an alkyne-modified isoprenoid analogue. Sci Rep. 2016 Dec 7;6:3861. PMID: 27924931. |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Gisselberg JE, Zhang L, Elias JE, Yeh E. The Prenylated Proteome of Plasmodium falciparum Reveals Pathogen-specific Prenylation Activity and Drug Mechanism-of-action. Mol Cell Proteomics. 2017 16(4 suppl 1):S54-S64. PMID: 28040698 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Candidate prenyl transferase substrates from the P. falciparum 3D7 proteome, which contain canonical CaaX motifs and were identified by PrePS. |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
PFID |
|
|
Name |
C-term |
PrePS |
EC# |
Transcript |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23294 |
PF3D7_0910600 |
|
|
SNARE protein (putative) |
KKQCCSIM |
FT***; GGT-1*** |
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23301 |
PF3D7_1233900 |
|
|
Ulp1 protease (putative) |
ISQGCLVF |
FT*; GGT-1* |
3.4.22.68 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23312 |
PF3D7_1460100 |
|
|
FYVE and coiled-coil domain-containing protein |
QRRMCNIM |
FT*; GGT-1** |
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 25932 |
PF3D7_1319100 |
|
|
ubiquitin carboxyl-terminal hydrolase MINDY, putative |
KKKKCTIM |
FT***; GGT-1*** |
3.4.19.12 |
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23914 |
PF3D7_1437900 |
|
|
Hsp40 subfamily A (putative) |
GRVACAQQ |
FT** |
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23306 |
PF3D7_1324700 |
|
|
SNARE protein (putative) |
NNQCCSLY |
FT* |
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23305 |
PF3D7_1323700 |
|
|
glideosome associated protein with multiple membrane spans 1 |
NIAACKQC |
FT* |
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23302 |
PF3D7_1311600 |
|
|
Uncharacterized protein |
NRLKCNIM |
GGT-1** |
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 42159 |
PF3D7_0322000 |
|
|
peptidyl-prolyl cis-trans isomerase |
CGEL |
FT– GGT1– |
5.2.1.8 |
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
PrePS was used to predict the likelihood of prenylation of the proteins identified: *low, **intermediate, ***high. Colored residues indicate putative sites for prenylation. |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
PrePS - Prenylation Prediction Suite |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Candidate prenyl transferase substrates from the P. falciparum 3D7 proteome, which were not identified by PrePS but possess additional, promising CaaX features. |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
PFID |
|
|
Name |
C-term |
PrePS |
EC# |
Transcript |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23295 |
PF3D7_1113100 |
|
|
Protein tyrosine phosphatase |
NCLRKCHFM |
|
3.1.3.48 |
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 34016 |
PF3D7_1428700 |
|
|
heme/steroid binding domain containing protein, putative |
KKRNKCNFM |
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23289 |
PF3D7_0525800 |
|
|
Membrane skeletal protein IMC1-related |
QRNLYCSYA |
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23287 |
PF3D7_0513600 |
|
|
Deoxyribodipyrimidine photolyase |
KREKKCVAS |
|
4.1.99.3 |
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23284 |
PF3D7_0302100 |
|
|
Ser/Thr protein kinase |
NKKNSCAYT |
|
2.7.11.1 |
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23285 |
PF3D7_0317700 |
|
|
CPSF subunit A |
NYNFLCLYI |
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Colored residues indicate putative sites for prenylation. |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Candidate prenyl transferase substrates from the P. falciparum 3D7 proteome, which possess –CXC and –CC C-terminal motifs for possible geranylgeranylation. |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
PFID |
|
|
Name |
C-term |
PrePS |
EC# |
Transcript |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
-CXC |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23311 |
PF3D7_1439000 |
|
|
Copper transporter putative |
ADPACCGC |
No |
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23308 |
PF3D7_1404300 |
|
|
Secreted ookinete adhesive protein |
ECSCSCSC |
No |
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23299 |
PF3D7_1144900 |
|
|
Rab6 |
NMLSKCLC |
GGT-2** |
3.6.5.2 |
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23292 |
PF3D7_0807300 |
|
|
Rab18 |
ESRSNCAC |
GGT-2*** |
3.6.5.2 |
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23288 |
PF3D7_0513800 |
|
|
Rab1a |
SPQSFCSC |
GGT-2*** |
3.6.5.2 |
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
-CC |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 27556 |
PF3D7_1140500 |
|
|
Myosin heavy chain subunit, putative |
ELNMFKCC |
No |
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23290 |
PF3D7_0608600 |
|
|
Conserved Plasmodium protein |
IKKKKMRCC |
No |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23296 |
PF3D7_1131500 |
|
|
Conserved Plasmodium protein |
TKKFFPCC |
No |
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23297 |
PF3D7_1140400 |
|
|
Conserved Plasmodium protein |
KTKKCYCC |
No |
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23484 |
PF3D7_0801400 |
|
|
von Willebrand factor A domain-related protein |
GKRFLGCC |
No |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23286 |
PF3D7_0512600 |
|
|
Rab1b |
KDTKKKCC |
GGT-2*** |
3.6.5.2 |
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23283 |
PF3D7_0211200 |
|
|
Rab5a |
TLSKKGCC |
GGT-2*** |
3.6.5.2 |
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23313 |
PF3D7_0106800 |
|
|
ras-related protein Rab-5C |
EETKKKCC |
GGT-2*** |
3.6.5.2 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23304 |
PF3D7_1320600 |
|
|
Rab11a |
TKKKNKCC |
GGT-2*** |
3.6.5.2 |
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23300 |
PF3D7_1231100 |
|
|
Rab2 |
SRSGFSCC |
GGT-2*** |
3.6.5.2 |
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23293 |
PF3D7_0903200 |
|
|
Rab7 |
KMYKSRCC |
GGT-2*** |
3.6.5.2 |
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23307 |
PF3D7_1340700 |
|
|
Rab11b |
NMNKVKCC |
GGT-2** |
3.6.5.2 |
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 23292 |
PF3D7_0807300 |
|
|
Ras related protein, Rab18 |
NCAC |
RabGGT+++ |
3.6.5.2 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 42160 |
PF3D7_1310600 |
|
|
Ras related protein, Rab5b |
|
RabGGT – |
3.6.5.2 |
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
PrePS was used to predict the likelihood of prenylation of the proteins identified: *low, **intermediate, ***high. Colored residues indicate putative sites for prenylation. |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|