Prenylated proteins
Suazo KF, Schaber C, Palsuledesai CC, Odom John AR, Distefano MD. Global proteomic analysis of prenylated proteins in Plasmodium falciparum using an alkyne-modified isoprenoid analogue. Sci Rep. 2016 Dec 7;6:3861. PMID: 27924931.
Gisselberg JE, Zhang L, Elias JE, Yeh E. The Prenylated Proteome of Plasmodium falciparum Reveals Pathogen-specific Prenylation Activity and Drug Mechanism-of-action. Mol Cell Proteomics. 2017 16(4 suppl 1):S54-S64. PMID: 28040698
Candidate prenyl transferase substrates from the P. falciparum 3D7 proteome, which contain canonical CaaX motifs and were identified by PrePS.
PFID Name C-term PrePS EC# Transcript
PF3D7_0910600 SNARE protein (putative) KKQCCSIM FT***; GGT-1***
PF3D7_1233900 Ulp1 protease (putative) ISQGCLVF FT*; GGT-1* 3.4.22.68
PF3D7_1460100 FYVE and coiled-coil domain-containing protein QRRMCNIM FT*; GGT-1**
PF3D7_1319100 ubiquitin carboxyl-terminal hydrolase MINDY, putative KKKKCTIM FT***; GGT-1*** 3.4.19.12
PF3D7_1437900 Hsp40 subfamily A (putative) GRVACAQQ FT**
PF3D7_1324700 SNARE protein (putative) NNQCCSLY FT*
PF3D7_1323700 glideosome associated protein with multiple membrane spans 1 NIAACKQC FT*
PF3D7_1311600 Uncharacterized protein NRLKCNIM GGT-1**
PF3D7_0322000 peptidyl-prolyl cis-trans isomerase CGEL  FT– GGT1– 5.2.1.8
PrePS was used to predict the likelihood of prenylation of the proteins identified: *low, **intermediate, ***high. Colored residues indicate putative sites for prenylation.
PrePS - Prenylation Prediction Suite
Candidate prenyl transferase substrates from the P. falciparum 3D7 proteome, which were not identified by PrePS but possess additional, promising CaaX features.
PFID Name C-term PrePS EC# Transcript
PF3D7_1113100 Protein tyrosine phosphatase NCLRKCHFM 3.1.3.48
PF3D7_1428700 heme/steroid binding domain containing protein, putative KKRNKCNFM
PF3D7_0525800 Membrane skeletal protein IMC1-related QRNLYCSYA
PF3D7_0513600 Deoxyribodipyrimidine photolyase KREKKCVAS 4.1.99.3
PF3D7_0302100 Ser/Thr protein kinase NKKNSCAYT 2.7.11.1
PF3D7_0317700 CPSF subunit A NYNFLCLYI
Colored residues indicate putative sites for prenylation.
Candidate prenyl transferase substrates from the P. falciparum 3D7 proteome, which possess –CXC and –CC C-terminal motifs for possible geranylgeranylation.
PFID Name C-term PrePS EC# Transcript
-CXC
PF3D7_1439000 Copper transporter putative ADPACCGC No
PF3D7_1404300 Secreted ookinete adhesive protein ECSCSCSC No
PF3D7_1144900 Rab6 NMLSKCLC GGT-2** 3.6.5.2
PF3D7_0807300 Rab18 ESRSNCAC GGT-2*** 3.6.5.2
PF3D7_0513800 Rab1a SPQSFCSC GGT-2*** 3.6.5.2
-CC
PF3D7_1140500 Myosin heavy chain subunit, putative ELNMFKCC No
PF3D7_0608600 Conserved Plasmodium protein IKKKKMRCC No
PF3D7_1131500 Conserved Plasmodium protein TKKFFPCC No
PF3D7_1140400 Conserved Plasmodium protein KTKKCYCC No
PF3D7_0801400 von Willebrand factor A domain-related protein GKRFLGCC No
PF3D7_0512600 Rab1b KDTKKKCC GGT-2*** 3.6.5.2
PF3D7_0211200 Rab5a TLSKKGCC GGT-2*** 3.6.5.2
PF3D7_0106800  ras-related protein Rab-5C EETKKKCC GGT-2*** 3.6.5.2
PF3D7_1320600 Rab11a TKKKNKCC GGT-2*** 3.6.5.2
PF3D7_1231100 Rab2 SRSGFSCC GGT-2*** 3.6.5.2
PF3D7_0903200 Rab7 KMYKSRCC GGT-2*** 3.6.5.2
PF3D7_1340700 Rab11b NMNKVKCC GGT-2** 3.6.5.2
PF3D7_0807300  Ras related protein, Rab18 NCAC RabGGT+++ 3.6.5.2
PF3D7_1310600 Ras related protein, Rab5b RabGGT – 3.6.5.2
PrePS was used to predict the likelihood of prenylation of the proteins identified: *low, **intermediate, ***high. Colored residues indicate putative sites for prenylation.
Online articles related to Prenylated proteins retrieved from PubMed

Related Maps