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Prenylated proteins |
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Suazo KF, Schaber C, Palsuledesai CC, Odom John AR, Distefano MD. Global proteomic analysis of prenylated proteins in Plasmodium falciparum using an alkyne-modified isoprenoid analogue. Sci Rep. 2016 Dec 7;6:3861. PMID: 27924931. |
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Gisselberg JE, Zhang L, Elias JE, Yeh E. The Prenylated Proteome of Plasmodium falciparum Reveals Pathogen-specific Prenylation Activity and Drug Mechanism-of-action. Mol Cell Proteomics. 2017 16(4 suppl 1):S54-S64. PMID: 28040698 |
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Candidate prenyl transferase substrates from the P. falciparum 3D7 proteome, which contain canonical CaaX motifs and were identified by PrePS. |
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PFID |
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Name |
C-term |
PrePS |
EC# |
Transcript |
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23294 |
PF3D7_0910600 |
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SNARE protein (putative) |
KKQCCSIM |
FT***; GGT-1*** |
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23301 |
PF3D7_1233900 |
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Ulp1 protease (putative) |
ISQGCLVF |
FT*; GGT-1* |
3.4.22.68 |
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23312 |
PF3D7_1460100 |
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FYVE and coiled-coil domain-containing protein |
QRRMCNIM |
FT*; GGT-1** |
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25932 |
PF3D7_1319100 |
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ubiquitin carboxyl-terminal hydrolase MINDY, putative |
KKKKCTIM |
FT***; GGT-1*** |
3.4.19.12 |
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23914 |
PF3D7_1437900 |
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Hsp40 subfamily A (putative) |
GRVACAQQ |
FT** |
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23306 |
PF3D7_1324700 |
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SNARE protein (putative) |
NNQCCSLY |
FT* |
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23305 |
PF3D7_1323700 |
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glideosome associated protein with multiple membrane spans 1 |
NIAACKQC |
FT* |
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23302 |
PF3D7_1311600 |
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Uncharacterized protein |
NRLKCNIM |
GGT-1** |
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42159 |
PF3D7_0322000 |
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peptidyl-prolyl cis-trans isomerase |
CGEL |
FT– GGT1– |
5.2.1.8 |
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PrePS was used to predict the likelihood of prenylation of the proteins identified: *low, **intermediate, ***high. Colored residues indicate putative sites for prenylation. |
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PrePS - Prenylation Prediction Suite |
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Candidate prenyl transferase substrates from the P. falciparum 3D7 proteome, which were not identified by PrePS but possess additional, promising CaaX features. |
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PFID |
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Name |
C-term |
PrePS |
EC# |
Transcript |
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23295 |
PF3D7_1113100 |
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Protein tyrosine phosphatase |
NCLRKCHFM |
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3.1.3.48 |
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34016 |
PF3D7_1428700 |
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heme/steroid binding domain containing protein, putative |
KKRNKCNFM |
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23289 |
PF3D7_0525800 |
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Membrane skeletal protein IMC1-related |
QRNLYCSYA |
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23287 |
PF3D7_0513600 |
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Deoxyribodipyrimidine photolyase |
KREKKCVAS |
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4.1.99.3 |
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23284 |
PF3D7_0302100 |
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Ser/Thr protein kinase |
NKKNSCAYT |
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2.7.11.1 |
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23285 |
PF3D7_0317700 |
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CPSF subunit A |
NYNFLCLYI |
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Colored residues indicate putative sites for prenylation. |
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Candidate prenyl transferase substrates from the P. falciparum 3D7 proteome, which possess –CXC and –CC C-terminal motifs for possible geranylgeranylation. |
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PFID |
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Name |
C-term |
PrePS |
EC# |
Transcript |
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-CXC |
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23311 |
PF3D7_1439000 |
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Copper transporter putative |
ADPACCGC |
No |
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23308 |
PF3D7_1404300 |
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Secreted ookinete adhesive protein |
ECSCSCSC |
No |
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23299 |
PF3D7_1144900 |
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Rab6 |
NMLSKCLC |
GGT-2** |
3.6.5.2 |
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23292 |
PF3D7_0807300 |
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Rab18 |
ESRSNCAC |
GGT-2*** |
3.6.5.2 |
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23288 |
PF3D7_0513800 |
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Rab1a |
SPQSFCSC |
GGT-2*** |
3.6.5.2 |
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-CC |
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27556 |
PF3D7_1140500 |
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Myosin heavy chain subunit, putative |
ELNMFKCC |
No |
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23290 |
PF3D7_0608600 |
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Conserved Plasmodium protein |
IKKKKMRCC |
No |
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23296 |
PF3D7_1131500 |
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Conserved Plasmodium protein |
TKKFFPCC |
No |
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23297 |
PF3D7_1140400 |
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Conserved Plasmodium protein |
KTKKCYCC |
No |
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23484 |
PF3D7_0801400 |
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von Willebrand factor A domain-related protein |
GKRFLGCC |
No |
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23286 |
PF3D7_0512600 |
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Rab1b |
KDTKKKCC |
GGT-2*** |
3.6.5.2 |
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23283 |
PF3D7_0211200 |
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Rab5a |
TLSKKGCC |
GGT-2*** |
3.6.5.2 |
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23313 |
PF3D7_0106800 |
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ras-related protein Rab-5C |
EETKKKCC |
GGT-2*** |
3.6.5.2 |
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23304 |
PF3D7_1320600 |
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Rab11a |
TKKKNKCC |
GGT-2*** |
3.6.5.2 |
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23300 |
PF3D7_1231100 |
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Rab2 |
SRSGFSCC |
GGT-2*** |
3.6.5.2 |
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23293 |
PF3D7_0903200 |
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Rab7 |
KMYKSRCC |
GGT-2*** |
3.6.5.2 |
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23307 |
PF3D7_1340700 |
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Rab11b |
NMNKVKCC |
GGT-2** |
3.6.5.2 |
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23292 |
PF3D7_0807300 |
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Ras related protein, Rab18 |
NCAC |
RabGGT+++ |
3.6.5.2 |
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42160 |
PF3D7_1310600 |
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Ras related protein, Rab5b |
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RabGGT – |
3.6.5.2 |
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PrePS was used to predict the likelihood of prenylation of the proteins identified: *low, **intermediate, ***high. Colored residues indicate putative sites for prenylation. |
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