Proteome of the parasitophorous vacuole
based on proteins interacting with PfEMP1 in the parasitophorous vacuole and in the host cytosol
Batinovic S, McHugh E, Chisholm SA, Matthews K, Liu B, Dumont L, Charnaud SC, Schneider MP, Gilson PR, de Koning-Ward TF, Dixon MWA, Tilley L. An exported protein-interacting complex involved in the trafficking of virulence determinants in Plasmodium-infected erythrocytes. Nat Commun. 2017 0;8:16044. PMID: 28691708
Color legend EPIC (Exported Protein-Interacting Complex)
PTEX
Parasite surface (PPM)
Secreted (PV lumen/PVM)
Exported protein (Transit through PV)
Unknown
Parasite proteins that are unique to, or ≥ 5-fold enriched in, PfEMP1B co-IP ((PV)-located ) compared to control PfEMP1F.
PfID Avg fold change E.C Transcript
8275 ­- >196
28400 PF3D7_1024800
exported protein 3 >26.25
28405 PF3D7_1116800
heat shock protein 101 chaperone protein ClpB2 >18
28428 PF3D7_1436300
translocon component PTEX150 >11.75
28407 PF3D7_1129100
parasitophorous vacuolar protein 1 10
28375 PF3D7_0629200
DnaJ protein, putative >8.25
28377 PF3D7_0721100
conserved Plasmodium protein, unknown function >7.5
28403 PF3D7_1105600
translocon component PTEX88 >5.25
28395 PF3D7_0936300
ring-exported protein 3 >4
28399 PF3D7_1016400
serine/threonine protein kinase, FIKK family >3.5 2.7.11.1
28371 PF3D7_0501200
parasite-infected erythrocyte surface protein >2
28410 PF3D7_1226900
parasitophorous vacuolar protein 2 >2
28408 PF3D7_1135400 hotdog domain-containing protein, putative >2
28415 PF3D7_1311800
M1-family alanyl aminopeptidase >2 3.4.11.2
28372 PF3D7_0501300
skeleton-binding protein 1 >2
28376 PF3D7_0716300 conserved Plasmodium protein, unknown function >2
28364 PF3D7_0112800
Plasmodium exported protein (hyp11), unknown function >1.75
28393 PF3D7_0935900
ring-exported protein 1 >1.75
28432 PF3D7_1471100
exported protein 2 >1.25
28389 PF3D7_0925900
lipocalin, lcn >1.25
28390 PF3D7_0928600 conserved Plasmodium protein, unknown function >1.25
28420 PF3D7_1345100
thioredoxin 2 >1.25
28423 PF3D7_1370300
membrane associated histidine-rich protein >1.25
28397 PF3D7_1002100
EMP1-trafficking protein >1
28382 PF3D7_0831400 Plasmodium exported protein, unknown function >1
28421 PF3D7_1353200
membrane associated histidine-rich protein 2 >1
28412 PF3D7_1239700
ATP-dependent zinc metalloprotease FTSH 1 >0.75 3.4.24.-
28401 PF3D7_1033200
early transcribed membrane protein 10.2 >0.5
Mass spectrometry analysis of PV1-HA interacting proteins Proteins that are unique to, or ≥ 5-fold enriched in, PV1-HA co-IP compared to control wildtype (WT)
28407 PF3D7_1129100
parasitophorous vacuolar protein 1 >104
28400 PF3D7_1024800
exported protein 3 >85.5
28428 PF3D7_1436300
translocon component PTEX150 >29
28383 PF3D7_0831700
heat shock protein 70 >22.75
28405 PF3D7_1116800
heat shock protein 101 chaperone protein ClpB2 >22.5
28427 PF3D7_1420700
surface protein P113 >17.25
28410 PF3D7_1226900
parasitophorous vacuolar protein 2 >12.5
28431 PF3D7_1464600
serine/threonine protein phosphatase uis2, putative >12 3.1.3.16
28411 PF3D7_1228600
merozoite surface protein 9 >10
28429 PF3D7_1454400
aminopeptidase P 9 3.4.11.9
28392 PF3D7_0930300
merozoite surface protein 1 >7.25
28403 PF3D7_1105600
translocon component PTEX88 >6.25
28384 PF3D7_0902800
serine repeat antigen 9 >5.25 3.4.22.2
28401 PF3D7_1033200
early transcribed membrane protein 10.2 >5
28377 PF3D7_0721100
conserved Plasmodium protein, unknown function >5
28391 PF3D7_0929400
high molecular weight rhoptry protein 2 >5
28398 PF3D7_1016300
glycophorin binding protein >4.75
28365 PF3D7_0207600
serine repeat antigen 5 >4.75 3.4.22.-
28415 PF3D7_1311800
M1-family alanyl aminopeptidase >4.75 3.4.11.2
28360 PF3D7_0104200
StAR-related lipid transfer protein >4.5
28432 PF3D7_1471100
exported protein 2 >4.5
28404 PF3D7_1116700
dipeptidyl aminopeptidase 1 cathepsin C, homolog >4.5 3.4.14.1
28378 PF3D7_0730900
EMP1-trafficking protein >3.75
28369 PF3D7_0406200
parasitophorous vacuole membrane protein s16; sexual stage-specific protein s16 >3
28376 PF3D7_0716300 conserved Plasmodium protein, unknown function >2.75
28371 PF3D7_0501200
parasite-infected erythrocyte surface protein >2.5
28399 PF3D7_1016400
serine/threonine protein kinase, FIKK family >2.5 2.7.11.1
28363 PF3D7_0112200
multidrug resistance-associated protein 1 MRP1 >2.5 3.6.3.44
33887 PF3D7_1104400
thioredoxin-like mero protein >2.5
28367 PF3D7_0215000 acyl-CoA synthetase >2.25 6.2.1.3
28373 PF3D7_0532100
early transcribed membrane protein 5 >2.25
28374 PF3D7_0532400
lysine-rich membrane-associated PHISTb protein >2.25
28430 PF3D7_1462300
GTP-binding protein, putative >2.25
28408 PF3D7_1135400 hotdog domain-containing protein, putative >2
28418 PF3D7_1334500
MSP7-like protein >1.75
28366 PF3D7_0207800
serine repeat antigen 3 >1.75 3.4.22.-
28385 PF3D7_0905400
high molecular weight rhoptry protein 3 >1.75
28396 PF3D7_1001500
early transcribed membrane protein 10., ETRAMP10.1 >1.75
28413 PF3D7_1252700
Plasmodium exported protein (PHISTb), unknown function >1.75
28425 PF3D7_1408000
plasmepsin II >1.5 3.4.23.39
28380 PF3D7_0829200 prohibitin, putative >1.5
28389 PF3D7_0925900
lipocalin, lcn >1.25
28426 PF3D7_1419200 thioredoxin-like protein, putative >1.25
28394 PF3D7_0936000
ring-exported protein 2 >1.25
28381 PF3D7_0830400
cra domain-containing protein, putative >1.25
28406 PF3D7_1121600
parasitophorous vacuole membrane antigen QF 116 exported protein 1 circumsporozoite-related antigen >1.25
28379 PF3D7_0811600 conserved Plasmodium protein, unknown function >1
28387 PF3D7_0912400
alkaline phosphatase, putative >1 3.1.3.1
28370 PF3D7_0500800
erythrocyte membrane protein 2 mature parasite-infected erythrocyte surface antigen >1
28419 PF3D7_1335100
merozoite surface protein 7 >0.75
28362 PF3D7_0104500 conserved protein, unknown function >0.75
28361 PF3D7_0104400
4-hydroxy-3-methylbut-2-enyl diphosphate reductase >0.75 1.17.1.2
28422 PF3D7_1364100
6-cysteine protein p92 >0.75
28388 PF3D7_0913700 conserved protein, unknown function >0.75
Proteins that are unique to, or ≥ 5-fold enriched in, PV2-HA co-IP compared to control wildtype (WT).
28410 PF3D7_1226900
parasitophorous vacuolar protein 2 >41.75
28407 PF3D7_1129100
parasitophorous vacuolar protein 1 19.5
28400 PF3D7_1024800
exported protein 3 >12.00
28405 PF3D7_1116800
heat shock protein 101 chaperone protein ClpB2 >3.25
28409 PF3D7_1201000
Plasmodium exported protein (PHISTb), unknown function >3.00
28411 PF3D7_1228600
merozoite surface protein 9 >1.75
28428 PF3D7_1436300
translocon component PTEX150 >1.75
28371 PF3D7_0501200
parasite-infected erythrocyte surface protein >1.67
28403 PF3D7_1105600
translocon component PTEX88 >1.25
28399 PF3D7_1016400
serine/threonine protein kinase, FIKK family >1.00 2.7.11.1
28432 PF3D7_1471100
exported protein 2 >0.75
Proteins that are unique to, or ≥ 5-fold enriched in, PV2-HA co-IP compared to control wildtype (WT).
28400 PF3D7_1024800
exported protein 3 >84.25
28407 PF3D7_1129100
parasitophorous vacuolar protein 1 >40.75
28428 PF3D7_1436300
translocon component PTEX150 >9
28410 PF3D7_1226900
parasitophorous vacuolar protein 2 >7
28404 PF3D7_1116700
dipeptidyl aminopeptidase 1 cathepsin C, homolog >5.5 3.4.14.1
28405 PF3D7_1116800
heat shock protein 101 chaperone protein ClpB2 >5.25
28429 PF3D7_1454400
aminopeptidase P >4.5 3.4.11.9
28384 PF3D7_0902800
serine repeat antigen 9 >3 3.4.22.2
28398 PF3D7_1016300