Peptidases and proteases

Peptidases and proteases

Weißbach T, Golzmann A, Bennink S, Pradel G, Julius Ngwa C. Transcript and protein expression analysis of proteases in the blood stages of Plasmodium falciparum. Exp Parasitol. 2017 180:33-44. PMID: 28351685.

Data for MEROPS were taken from (http://merops.sanger.ac.uk/index.htm) with additions. Clans and families are linked to MEROPS.

PfID

Annotation

EC

Transcript

MEROPS assignment

Cysteine proteases

Clan

Family

MEROPS ID

cysteine proteinase falcipain 1

cysteine proteinase falcipain 2a falcipain 2

falcipain 2' cysteine proteinase falcipain 2b

cysteine proteinase falcipain 3

dipeptidyl aminopeptidase 1 cathepsin C, homolog

dipeptidyl aminopeptidase 2

dipeptidyl aminopeptidase 3

serine repeat antigen 1

serine repeat antigen 2

serine repeat antigen 3

serine repeat antigen 4

serine repeat antigen 5

serine repeat antigen 6

serine repeat antigen 7

serine repeat antigen 8

serine repeat antigen 9

calpain

ubiquitin carboxyl-terminal hydrolase uch54

ubiquitin carboxyl-terminal hydrolase 1, putative

ubiquitin specific protease, putative

ubiquitin carboxyl-terminal hydrolase 13, putative

ubiquitin carboxyl-terminal hydrolase 14

ubiquitin carboxyl-terminal hydrolase 2, putative

ubiquitin carboxyl-terminal hydrolase, putative

ubiquitin specific protease, putative

U4/U6.U5 tri-snRNP-associated protein 2, putative

ubiquitin carboxyl-terminal hydrolase, putative

non-peptidase homologue

GPI-anchor transamidase

non-peptidase homologue

metacaspase 1

non-peptidase homologue

metacaspase-2

sentrin-specific protease 1

sentrin-specific protease 2, putative

protein DJ-1

asparagine synthetase [glutamine-hydrolyzing], putative

peptidase family C50, putative separase, putative

PPPDE peptidase, putative

para-aminobenzoic acid synthetase

PPPDE peptidase, putative

GMP synthase [glutamine-hydrolyzing]

glutamine--fructose-6-phosphate aminotransferase [isomerizing], putative

OTU-like cysteine protease

Josephin domain-containing protein, putative

pyridoxine biosynthesis protein PDX2

PPPDE peptidase domain-containing protein, putative

ataxin-3, putative

carbamoyl phosphate synthetase

CTP synthase

ubiquitin carboxyl-terminal hydrolase isozyme L3

Aspartic proteases

plasmepsin I

AA

A1

A01.022

plasmepsin II

plasmepsin III histo-aspartic protease

plasmepsin IV

NA

PEXEL protease plasmepsin V

plasmepsin VI

plasmepsin VII

plasmepsin viii, putative

plasmepsin IX

plasmepsin X

NA

microgamete surface protein migs, putative

DNA damage-inducible protein 1

signal peptide peptidase

Serine proteases

serine protease DegP

peptidase, putative

ATP-dependent Clp protease proteolytic subunit

ATP-dependent Clp protease proteolytic subunit

non-peptidase homologue

chaperone protein ClpB1

NA

ATP-dependent Clp protease regulatory subunit Clpc, putative

NA

heat shock protein 101 chaperone protein ClpB2

NA

ATP-dependent protease, putative

type I signal peptidase

non-peptidase homologue

signal peptidase complex catalytic subunit SEC11

subtilisin-like protease 1

subtilisin-like protease 2

subtilisin-like protease 3

non-peptidase homologue

rhomboid protease ROM1

rhomboid protease ROM3

rhomboid protease ROM4

rhomboid protease ROM6, putative

rhomboid protease ROM7

rhomboid protease ROM8

rhomboid protease ROM9

rhomboid protease ROM10

non-peptidase homologue

alpha/beta hydrolase, putative

trypsin-like serine protease, putative

NA

alpha/beta hydrolase, putative

NA

GTP-binding protein YihA3

NA

DER1-like protein, putative

non-peptidase homologue

proline aminopeptidase

alpha/beta hydrolase, putative

NA

derlin-1

non-peptidase homologue

exported lipase 1

alpha/beta hydrolase, putative gametocyte exported protein 8

alpha/beta hydrolase, putative

epoxide hydrolase 1

Threonine proteases

proteasome subunit alpha type-1, putative

proteasome subunit alpha type-2, putative

proteasome subunit alpha type-3, putative

proteasome subunit alpha type-4, putative

proteasome subunit alpha type-5, putative

proteasome subunit alpha type-6, putative

proteasome subunit alpha type-7, putative

proteasome subunit beta type-1, putative

proteasome subunit beta type-4

proteasome subunit beta type-3, putative

NA

proteasome subunit beta type-2, putative

proteasome subunit beta type-6, putative

proteasome subunit beta type-7, putative

proteasome subunit beta type-5

ATP-dependent protease subunit ClpQ

Metalloproteases

M1-family alanyl aminopeptidase

peptidase

mitochondrial intermediate peptidase, putative

cytochrome b-c1 complex subunit 2, putative

non-peptidase homologue

cytochrome b-c1 complex subunit 1, putative

insulinase, putative

peptidase m16, putative

non-peptidase homologue

falcilysin

stromal-processing peptidase, putative

M17 leucyl aminopeptidase

M18 aspartyl aminopeptidase

tRNA N6-adenosine threonylcarbamoyltransferase

NA

methionine aminopeptidase 1c, putative

methionine aminopeptidase 1b, putative

methionine aminopeptidase 2

aminopeptidase P

ATP-dependent zinc metalloprotease FTSH 1

ATP-dependent zinc metalloprotease FTSH, putative

ATP-dependent zinc metalloprotease FTSH, putative

zinc-carboxypeptidase, putative

FACT complex subunit SPT16, putative

non-peptidase homologue

methionine aminopeptidase 1a, putative

26S proteasome regulatory subunit RPN8, putative

apicoplast-minus ipp-rescued 4

eukaryotic translation initiation factor 3 subunit F, putative

non-peptidase homologue

DnaJ protein, putative

NA

metalloprotease

site-2 protease s2p, putative

26S proteasome regulatory subunit RPN11, putative

proliferation-associated protein 2g4, putative

non-peptidase homologue

mitochondrial inner membrane protease atp23, putative

M1-family alanyl aminopeptidase, putative

non-peptidase homologue

Online articles related to Peptidases and proteases retrieved from PubMed

Drugs affecting pathway with unknown specific target

Drug Name	PubMed Articles (year of publication)
Amprenavir	Antimalarial activity of human immunodeficiency virus type 1 protease inhibitors
Atazanavir	Antimalarial activity of human immunodeficiency virus type 1 protease inhibitors
Benzyloxycarbonyl-phenylalanyl-alanyl diazomethylketone	Analysis of antimalarial synergy between bestatin and endoprotease inhibitors using statistical response-surface modelling
Bestatin	Analysis of antimalarial synergy between bestatin and endoprotease inhibitors using statistical response-surface modelling
E-64	Analysis of antimalarial synergy between bestatin and endoprotease inhibitors using statistical response-surface modelling
Indinavir	Antimalarial activity of human immunodeficiency virus type 1 protease inhibitors
Lopinavir	Antimalarial activity of human immunodeficiency virus type 1 protease inhibitors
Nelfinavir	Antimalarial activity of human immunodeficiency virus type 1 protease inhibitors
Pepstatin	Analysis of antimalarial synergy between bestatin and endoprotease inhibitors using statistical response-surface modelling
Ritonavir	Antimalarial activity of human immunodeficiency virus type 1 protease inhibitors Synergistic interactions of the antiretroviral protease inhibitors saquinavir and ritonavir with chloroquine and mefloquine against Plasmodium falciparum in vitro
Saquinavir	Antimalarial activity of human immunodeficiency virus type 1 protease inhibitors Synergistic interactions of the antiretroviral protease inhibitors saquinavir and ritonavir with chloroquine and mefloquine against Plasmodium falciparum in vitro