Essential genes of parasitic apicomplexa - P. falciparum
Oberstaller J, Otto TD, Rayner JC, Adams JH. Essential Genes of the Parasitic Apicomplexa. Trends Parasitol. 2021 Jan 5:S1471-4922(20)30322-6.  PMID: 33419671.
Genes that tolerate disruption of their protein-CoDing Sequence (CDS) are dispensable whereas parasites die when the CDS of essential genes are disrupted, meaning that parasites with mutations in these genes are not recovered
Functional category PfID Annotation Mutability EC# Transcript
DNA metabolism and replication PF3D7_0206000 DNA repair protein RAD2, putative Mutable in CDS 4.2.99.18
PF3D7_0215800 origin recognition complex subunit 5 Non - Mutable in CDS
PF3D7_0216000 DEAD/DEAH box helicase, putative Mutable in CDS 3.6.4.13
PF3D7_0219600
replication factor C subunit 1, putative Non - Mutable in CDS
PF3D7_0305600
AP endonuclease (DNA-[apurinic or apyrimidinic site] lyase), putative Non - Mutable in CDS 4.2.99.18
PF3D7_0504400
ATP-dependent helicase, putative Non - Mutable in CDS 3.6.1.3
PF3D7_0605800
DNA repair protein RAD50, putative Mutable in CDS
PF3D7_0606500 polypyrimidine tract binding protein, putative Mutable in CDS
PF3D7_0625600 poly(A) polymerase PAP, putative Non - Mutable in CDS 2.7.7.19
PF3D7_0705400 DNA replication licensing factor MCM7 Non - Mutable in CDS
PF3D7_0726300 DNA mismatch repair protein PMS1, putative Non - Mutable in CDS
PF3D7_0910900 DNA primase large subunit, putative Non - Mutable in CDS 2.7.7.6
PF3D7_1014400
MO15-related protein kinase Non - Mutable in CDS 2.7.11.22
PF3D7_1017000
DNA polymerase delta catalytic subunit Non - Mutable in CDS 2.7.7.7
PF3D7_1106700 DNA replication ATP-dependent helicase/nuclease dna2, putative Mutable in CDS 3.6.4.12
PF3D7_1111100 replication factor C subunit 5, putative Non - Mutable in CDS
PF3D7_1120600 ddrgk domain-containing protein, putative Mutable in CDS
PF3D7_1129500 A/G-specific adenine glycosylase, putative Mutable in CDS 3.2.2.20
PF3D7_1216900 DNA-binding chaperone, putative Non - Mutable in CDS
PF3D7_1224300
polyadenylate-binding protein 1, putative Non - Mutable in CDS
PF3D7_1234300 DNA polymerase epsilon subunit B, putative Non - Mutable in CDS 2.7.7.7
PF3D7_1239500
DNA gyrase subunit B Non - Mutable in CDS 5.99.1.3
PF3D7_1250800 DNA repair protein rhp16, putative Mutable in CDS
PF3D7_1304100
DNA ligase I Non - Mutable in CDS 6.5.1.1
PF3D7_1305000 MCL1 domain-containing protein, putative Non - Mutable in CDS
PF3D7_1317100 DNA replication licensing factor MCM4 Non - Mutable in CDS
PF3D7_1338400 sprt-like domain-containing protein, putative Mutable in CDS
PF3D7_1343400 DNA repair protein RAD5, putative Mutable in CDS 3.6.4.12
PF3D7_1355100
DNA replication licensing factor MCM6 Non - Mutable in CDS
PF3D7_1365600
DNA topoisomerase 6 subunit b, putative Mutable in CDS
PF3D7_1409300 DNA damage-inducible protein 1, putative Non - Mutable in CDS 3.4.23.-
PF3D7_1417800
DNA replication licensing factor MCM2 Non - Mutable in CDS 3.6.4.12
PF3D7_1438500
cleavage and polyadenylation specificity factor subunit 3, putative Non - Mutable in CDS
PF3D7_1438700 DNA primase small subunit Non - Mutable in CDS 2.7.7.6
PF3D7_1463300 DNA polymerase alpha subunit B, putative Non - Mutable in CDS 2.7.7.7
PF3D7_1474300 DNA repair metallo-beta-lactamase protein, putative Mutable in CDS 3.5.2.6
RNA metabolism and transcription PF3D7_0107200
ccr4 domain-containing protein 3, putative Mutable in CDS
PF3D7_0203700 protein MAK16, putative Non - Mutable in CDS
PF3D7_0209800
ATP-dependent RNA helicase UAP56 Non - Mutable in CDS 3.6.4.13
PF3D7_0218700 pre-mRNA-processing protein 45, putative Non - Mutable in CDS
PF3D7_0303100 CLP1 P-loop domain-containing protein. Mutable in CDS
PF3D7_0303300 DNA-directed RNA polymerases I, II, and III subunit RPABC2, putative Non - Mutable in CDS 2.7.7.6
PF3D7_0308100 zinc finger protein, putative Mutable in CDS
PF3D7_0308600 pre-mRNA-processing factor 19, putative Non - Mutable in CDS
PF3D7_0320800
ATP-dependent RNA helicase DDX6 Mutable in CDS 3.6.4.13
PF3D7_0403700 pre-mRNA-splicing factor CLF1, putative Mutable in CDS
PF3D7_0408900
tRNA N6-adenosine threonylcarbamoyltransferase Non - Mutable in CDS 2.6.99.4
PF3D7_0409100 U4/U6 small nuclear ribonucleoprotein PRP31, putative Non - Mutable in CDS
PF3D7_0410400
exosome complex component RRP4, putative Non - Mutable in CDS
PF3D7_0414500 RNA-binding protein, putative Mutable in CDS
PF3D7_0417500 memo-like protein Non - Mutable in CDS
PF3D7_0506800 transcription factor 25, putative Mutable in CDS
PF3D7_0508700 pre-mRNA-processing ATP-dependent RNA helicase PRP5, putative Non - Mutable in CDS 3.6.4.13
PF3D7_0509400
RNA polymerase I Non - Mutable in CDS 2.7.7.6
PF3D7_0511500 RNA pseudouridylate synthase, putative Mutable in CDS 5.4.99.12
PF3D7_0515000 pre-mRNA-splicing factor CWC2, putative Mutable in CDS
PF3D7_0516800 AP2 domain transcription factor AP2-O2, putative Mutable in CDS
PF3D7_0517900 zinc finger protein, putative Non - Mutable in CDS
PF3D7_0519500 ccr4 domain-containing protein 1, putative Mutable in CDS
PF3D7_0524900 S-adenosyl-l-methionine-dependent trna 4-demethylwyosine synthase, putative Mutable in CDS 4.1.3.44
PF3D7_0528100 AP-1/2 complex subunit beta, putative Non - Mutable in CDS
PF3D7_0602000 zinc finger protein, putative Mutable in CDS
PF3D7_0607100 MYND finger protein, putative Mutable in CDS
PF3D7_0619900 splicing factor 3A subunit 2, putative Mutable in CDS
PF3D7_0622500 RNA methyltransferase, putative Mutable in CDS 2.1.1.-
PF3D7_0622900
AP2 domain transcription factor AP2Tel Mutable in CDS
PF3D7_0623700 ATP-dependent RNA helicase suv3, putative Mutable in CDS 3.6.4.13
PF3D7_0630900 ATP-dependent RNA helicase HAS1 Non - Mutable in CDS 3.6.4.13
PF3D7_0705000 mrna cap guanine-n7 methyltransferase, putative Non - Mutable in CDS 2.1.1.56
PF3D7_0707600 mediator of RNA polymerase II transcription subunit 10 Mutable in CDS
PF3D7_0709600 ribonucleases P/MRP protein subunit POP1, putative Non - Mutable in CDS 3.1.26.5
PF3D7_0714500 transcription elongation factor s-II, putative Non - Mutable in CDS
PF3D7_0717400 queuine tRNA-ribosyltransferase, putative Mutable in CDS 2.4.2.29
PF3D7_0720000 exosome complex component CSL4, putative Non - Mutable in CDS 3.1.13.1
PF3D7_0729500 n6-adenosine-methyltransferase mt-a70; METTL3 Non - Mutable in CDS 2.1.1.62
PF3D7_0730100 tRNA pseudouridine synthase D, putative Mutable in CDS 5.4.99.12
PF3D7_0818000 U4/U6.U5 small nuclear ribonucleoprotein, putative Non - Mutable in CDS
PF3D7_0822200 phosphorylated ctd interacting factor 1 ww domain-containing protein, putative Mutable in CDS
PF3D7_0904100 AP-4 complex subunit epsilon, putative Non - Mutable in CDS
PF3D7_0909400 exoribonuclease, putative Non - Mutable in CDS 3.1.13.1
PF3D7_0917600
pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43, putative Non - Mutable in CDS 3.6.4.13
PF3D7_0920900 U4/U6 snRNA-associated-splicing factor, putative Non - Mutable in CDS
PF3D7_0922700 pre-mRNA-splicing factor 18, putative Non - Mutable in CDS
PF3D7_0925600 zinc binding protein (Yippee), putative Mutable in CDS
PF3D7_0934100
TFIIH basal transcription factor complex helicase XPD subunit Non - Mutable in CDS 3.6.4.12
PF3D7_1005500 regulator of nonsense transcripts 1, putative Mutable in CDS
PF3D7_1006000 IMP1-like protein, putative Mutable in CDS
PF3D7_1009200 ribonuclease, putative Non - Mutable in CDS
PF3D7_1011800 PRE-binding protein Non - Mutable in CDS
PF3D7_1012000 ring zinc finger protein, putative Mutable in CDS
PF3D7_1014600 transcriptional coactivator ADA2 Non - Mutable in CDS
PF3D7_1018600 trna wybutosine-synthesizing protein, putative Mutable in CDS 2.1.1.-
PF3D7_1027100 U3 small nucleolar ribonucleoprotein protein MPP10, putative Mutable in CDS
PF3D7_1027400 DNA-directed RNA polymerase II subunit RPB7, putative Non - Mutable in CDS 2.7.7.6
PF3D7_1030100
pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22, putative Non - Mutable in CDS 3.6.4.13
PF3D7_1035000 U2 snRNA/tRNA pseudouridine synthase, putative Non - Mutable in CDS 5.4.99.12
PF3D7_1107800
AP2 domain transcription factor, putative Non - Mutable in CDS
PF3D7_1110200 pre-mRNA-processing factor 6, putative Mutable in CDS
PF3D7_1115500 AP2 domain transcription factor, putative Non - Mutable in CDS
PF3D7_1119500 AP-4 complex subunit mu, putative Non - Mutable in CDS
PF3D7_1120500 tRNA nucleotidyltransferase, putative Non - Mutable in CDS 2.7.7.72
PF3D7_1129400 rRNA (cytosine-C(5))-methyltransferase, putative Non - Mutable in CDS 2.1.1.-
PF3D7_1133800
RNA (uracil-5-)methyltransferase, putative Non - Mutable in CDS 2.1.1.35
PF3D7_1134700 DNA-directed RNA polymerase I subunit RPA2, putative Non - Mutable in CDS 2.7.7.6
PF3D7_1139100 RNA-binding protein, putative Mutable in CDS
PF3D7_1206600 DNA-directed RNA polymerase III subunit RPC2, putative Mutable in CDS 2.7.7.6
PF3D7_1207100 pre-rRNA-processing protein esf1, putative Mutable in CDS
PF3D7_1207500 RNA-binding protein, putative Non - Mutable in CDS
PF3D7_1207700 41-3 protein Non - Mutable in CDS
PF3D7_1217200 multiple rna-binding domain-containing protein 1 Non - Mutable in CDS
PF3D7_1220100 pre-mRNA-processing factor 17, putative Non - Mutable in CDS
PF3D7_1222600
AP2 domain transcription factor AP-G Mutable in CDS
PF3D7_1226700 U3 small nucleolar RNA-interacting protein 2, putative Mutable in CDS
PF3D7_1227900 RNA pseudouridylate synthase, putative Non - Mutable in CDS 4.2.1.70
PF3D7_1228500 RNA pseudouridylate synthase, putative Non - Mutable in CDS 5.4.99.12
PF3D7_1241800 ATP-dependent RNA helicase DBP9, putative Non - Mutable in CDS 3.6.4.13
PF3D7_1244200 RNA polymerase II transcription factor B subunit 2, putative Non - Mutable in CDS 2.7.7.6
PF3D7_1305200 AP2 domain transcription factor, putative Mutable in CDS
PF3D7_1306900 U1 small nuclear ribonucleoprotein A, putative Non - Mutable in CDS
PF3D7_1307000 exosome complex component RRP40, putative Mutable in CDS
PF3D7_1309300 U4/U6 small nuclear ribonucleoprotein PRP3, putative Non - Mutable in CDS
PF3D7_1311200 alternative splicing regulator, putative Non - Mutable in CDS
PF3D7_1311400
AP-1 complex subunit mu-1 Non - Mutable in CDS
PF3D7_1321700 splicing factor 1 Mutable in CDS
PF3D7_1326300 RNA-binding protein, putative Non - Mutable in CDS
PF3D7_1328800
transcriptional regulatory protein sir2a Mutable in CDS 3.5.1.98
PF3D7_1329000 DNA-directed RNA polymerase III subunit RPC1, putative Non - Mutable in CDS 2.7.7.6
PF3D7_1337300 exoribonuclease, putative Non - Mutable in CDS 3.1.16.1
PF3D7_1340100
exosome complex component RRP42, putative Non - Mutable in CDS 2.7.7.56
PF3D7_1340600 RNA lariat debranching enzyme, putative Non - Mutable in CDS 3.1.4.-
PF3D7_1343900 U4/U6 small nuclear ribonucleoprotein PRP4, putative Non - Mutable in CDS
PF3D7_1353500 RNA polymerase II transcription factor B subunit 4, putative Non - Mutable in CDS 2.7.7.6
PF3D7_1354300 large subunit rRNA methyltransferase, putative Non - Mutable in CDS 2.1.1.-
PF3D7_1357700 U3 small nucleolar RNA-associated protein 21, putative Non - Mutable in CDS
PF3D7_1359300
exosome complex exonuclease RRP44 Mutable in CDS 3.1.16.1
PF3D7_1362200
RuvB-like helicase 3 Mutable in CDS 3.6.4.12
PF3D7_1362500
3'-5' exonuclease, putative Non - Mutable in CDS 3.1.-.-
PF3D7_1363500
CCR4 domain-containing protein 2, putative Mutable in CDS
PF3D7_1364800 DNA-directed RNA polymerases I, II, and III subunit RPABC1, putative Non - Mutable in CDS 2.7.7.6
PF3D7_1367100 U1 small nuclear ribonucleoprotein 70 kda homolog, putative Non - Mutable in CDS
PF3D7_1407100
rRNA 2'-O-methyltransferase fibrillarin, putative Non - Mutable in CDS
PF3D7_1408900 tRNA-dihydrouridine synthase, putative Mutable in CDS 1.3.1.89
PF3D7_1417500 H/ACA ribonucleoprotein complex subunit 4, putative Non - Mutable in CDS 5.4.99.-
PF3D7_1421400 DNA-directed RNA polymerase III subunit RPC6, putative Non - Mutable in CDS 2.7.7.6
PF3D7_1428800 transcription initiation TFIID-like, putative Non - Mutable in CDS
PF3D7_1429000 protein archease, putative Non - Mutable in CDS
PF3D7_1437200
ribonucleoside-diphosphate reductase large subunit, putative Non - Mutable in CDS 1.17.4.1
PF3D7_1443500 poly(A)-specific ribonuclease PARN, putative Non - Mutable in CDS 3.1.13.4
PF3D7_1445600 RNA-binding protein, putative Mutable in CDS
PF3D7_1455500 AP-1 complex subunit gamma, putative Non - Mutable in CDS
PF3D7_1459000
ATP-dependent RNA helicase DBP5 Non - Mutable in CDS 3.6.4.13
PF3D7_1463400 DNA-directed RNA polymerase III subunit RPC4, putative Non - Mutable in CDS 2.7.7.6
PF3D7_1465200 mediator of RNA polymerase II transcription subunit 4 Non - Mutable in CDS