Essential, Non-mutable genes
Zhang M, Wang C, Otto TD, Oberstaller J, Liao X, Adapa SR, Udenze K, Bronner IF, Casandra D, Mayho M, Brown J, Li S, Swanson J, Rayner JC, Jiang RHY, Adams JH. Uncovering the essential genes of the human malaria parasite Plasmodium falciparum by saturation mutagenesis. Science. 2018 May 4;360(6388).  PMID: 29724925.
Mutagenesis index scores (MISs) and mutagenesis fitness scores (MFSs). Both the IS and MFS were predictive of the fitness cost for in vitro asexual growth. MIS is assigned on a scale of 0 to 1, with closer to 0 indicating a high probability of essentialit
Functional category PfID Annotation MIS MFS EC Transcript
39265 DNA metabolism PF3D7_0504400
ATP-dependent helicase, putative 0.119 -2.91 3.6.1.3
39697 PF3D7_1223300
DNA gyrase subunit A 0.119 -3.116 5.99.1.3
39474 PF3D7_0910900 DNA primase large subunit, putative 0.119 -3.17 2.7.7.6
39879 PF3D7_1368800 DNA repair endonuclease XPF, putative 0.119 -3.061 3.1.21.1
39274 PF3D7_0509500 ERCC4 domain-containing protein, putative 0.119 -3.048
39731 PF3D7_1241700
replication factor C subunit 4, putative 0.119 -3.256
39590 PF3D7_1106000
RuvB-like helicase 2 0.119 -3.016 3.6.4.12
39498 PF3D7_0924800 telomeric repeat binding factor 1, putative 0.119 -3.721
39307 PF3D7_0528100 AP-1/2 complex subunit beta, putative 0.12 -2.959
39974 PF3D7_1455500 AP-1 complex subunit gamma, putative 0.12 -3.152
39334 PF3D7_0613500 AP-3 complex subunit beta, putative 0.12 -3.089
39990 PF3D7_1463700
cyclin 1 0.12 -3.003
39894 PF3D7_1408400 FANCJ-like helicase, putative 0.12 -2.996 3.6.4.12
39843 PF3D7_1347500
DNA/RNA-binding protein Alba 4 0.12 -3.068
39641 PF3D7_1134700 DNA-directed RNA polymerase I subunit RPA2, putative 0.12 -2.941 2.7.7.6
39808 PF3D7_1329000 DNA-directed RNA polymerase III subunit RPC1, putative 0.12 -2.953 2.7.7.6
39160 PF3D7_0218000
replication factor C subunit 2, putative 0.12 -3.168
39602 PF3D7_1111100 replication factor C subunit 5, putative 0.12 -2.946
39277 PF3D7_0510500
topoisomerase I 0.12 -3.151 5.99.1.2
39448 PF3D7_0824100 3'-5' exonuclease, putative 0.121 -3.001 3.1.11.1
39468 PF3D7_0906000
exoribonuclease II 0.121 -3.052 3.1.27.1
39138 PF3D7_0112000 TatD-like deoxyribonuclease 0.121 -3.108 3.1.21.-
39916 PF3D7_1420200 myosin-specific chaperone unc, putative 0.121 -3.07
39358 PF3D7_0630300 DNA polymerase epsilon catalytic subunit A, putative 0.122 -3.04 2.7.7.7
39858 PF3D7_1355100
DNA replication licensing factor MCM6 0.122 -3.094
39604 PF3D7_1111600 endonuclease/exonuclease/phosphatase family protein, putative 0.122 -2.893 3.1.3.56
39954 PF3D7_1443500 poly(A)-specific ribonuclease PARN, putative 0.122 -3.034 3.1.13.4
39481 PF3D7_0915200 ribonuclease H2 subunit C, putative 0.122 -3.048 3.1.26.4
39664 PF3D7_1201500 XPA binding protein 1, putative 0.122 -3.045
39849 PF3D7_1352700 intron-binding protein aquarius, putative 0.123 -2.897
39382 PF3D7_0709600 ribonucleases P/MRP protein subunit POP1, putative 0.123 -3.036 3.1.26.5
39940 PF3D7_1438700 DNA primase small subunit 0.124 -3.197 2.7.7.6
39466 PF3D7_0904800 replication protein A1, small fragment 0.124 -2.834
39557 PF3D7_1027600 meiotic recombination protein spo11-2, putative 0.124 -3.101
39135 PF3D7_0109200 cleavage and polyadenylation specificity factor subunit 5 0.125 -3.001
39716 PF3D7_1234300 DNA polymerase epsilon subunit B, putative 0.125 -3.201 2.7.7.7
39211 PF3D7_0317400 DNA replication complex gins protein, putative 0.127 -2.853
39368 PF3D7_0705300
mediator of RNA polymerase ii transcription subunit 10, putative 0.127 -2.838
39547 PF3D7_1017000
DNA polymerase delta catalytic subunit 0.128 -3.097 2.7.7.7
39156 PF3D7_0215800 origin recognition complex subunit 5 0.128 -2.848
39191 PF3D7_0308000 DNA polymerase delta small subunit, putative 0.129 -2.834 2.7.7.7
39963 PF3D7_1449400 crossover junction endonuclease MUS81, putative 0.13 -2.842
39895 PF3D7_1409300
DNA damage-inducible protein 1 0.13 -2.861 3.4.23.-
39230 PF3D7_0411900 DNA polymerase alpha 0.13 -2.963 2.7.7.7
39480 PF3D7_0914800 GINS complex subunit Psf3, putative 0.13 -3.152
39907 PF3D7_1415000
uracil-DNA glycosylase 0.13 -3.285 3.2.2.27
39705 PF3D7_1226600
proliferating cell nuclear antigen 2 0.131 -3.303
39532 PF3D7_1010200 DNA2/NAM7 helicase, putative 0.132 -2.94 3.6.4.12
39605 PF3D7_1112600 ATPase, putative 0.134 -2.843
39989 PF3D7_1463300 DNA polymerase alpha subunit B, putative 0.135 -3.065 2.7.7.7
39688 PF3D7_1216900 DNA-binding chaperone, putative 0.137 -2.85
39473 PF3D7_0909400 exoribonuclease, putative 0.137 -2.944 3.1.13.1
39397 PF3D7_0720800 Ham1-like protein, putative 0.139 -2.831
39404 PF3D7_0726300 DNA mismatch repair protein PMS1, putative 0.141 -2.924
39732 RNA metabolism PF3D7_1241800 ATP-dependent RNA helicase DBP9, putative 0.119 -3.034 3.6.4.13
39359 PF3D7_0630900 ATP-dependent RNA helicase HAS1 0.119 -3.188 3.6.4.13
39666 PF3D7_1202000
ATP-dependent DNA/RNA helicase PSH2 0.119 -2.992 3.6.4.13
39268 PF3D7_0507600 protein caf40, putative 0.119 -3.091
39980 PF3D7_1458800 DNA-directed RNA polymerase III subunit RPC5, putative 0.119 -3.131
39964 PF3D7_1449700
exosome complex exonuclease RRP6 0.119 -3.009 3.1.13.-
39925 PF3D7_1429000 protein archease, putative 0.119 -2.982
39741 PF3D7_1244200 RNA polymerase II transcription factor B subunit 2, putative 0.119 -3.01 2.7.7.6
39244 PF3D7_0418700 RNA-binding protein NOB1, putative 0.119 -2.985
39225 PF3D7_0407300 transcription factor, putative 0.119 -3.016
39509 PF3D7_0935000 U2 small nuclear ribonucleoprotein B'', putative 0.119 -3.017
39539 PF3D7_1013100 U3 small nucleolar RNA-associated protein 13, putative 0.119 -3.035
39622 PF3D7_1124400 U6 snRNA-associated Sm-like protein LSm1, putative 0.119 -3.029
39915 PF3D7_1419900 YTH domain-containing protein 1, putative, YTH1 0.119 -2.98
39229 PF3D7_0409800 zinc finger protein, putative 0.119 -3.047
39991 PF3D7_1464200 zinc finger ccch domain-containing protein, putative 0.119 -2.986
39820 PF3D7_1337300 exoribonuclease, putative 0.12 -2.956 3.1.16.1
39999 PF3D7_1469700 mediator of RNA polymerase II transcription subunit 6, putative 0.12 -3.098
39568 PF3D7_1033600 pre-mRNA-splicing factor cef1, putative 0.12 -3.012
39269 PF3D7_0507700 nuclear protein localization protein 4, putative 0.12 -2.935
39869 PF3D7_1364300
pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 0.12 -2.978 3.6.4.13
39778 PF3D7_1311000
protein ISD11, putative 0.12 0 2.8.1.7
39851 PF3D7_1353500 RNA polymerase II transcription factor B subunit 4, putative 0.12 -2.95 2.7.7.6
39567 PF3D7_1032900
RNA polymerase II-associated protein 1, putative 0.12 -3.103
39424 PF3D7_0811900 RNA-binding protein, putative 0.12 -2.962
39634 PF3D7_1129400 RNA (cytosine-C(5))-methyltransferase, putative 0.12 -3.043 2.1.1.-
39632 PF3D7_1126900 small nuclear ribonucleoprotein F, putative 0.12 0
39445 PF3D7_0822300 small nuclear ribonucleoprotein G, putative 0.12 0
39793 PF3D7_1317900 nucleolar complex protein 4, putative 0.12 -3.009
39193 PF3D7_0308900 splicing factor 3B subunit 1, putative 0.12 -2.922
39620 PF3D7_1123900
13 kda ribonucleoprotein-associated protein, putative 0.12 -3.123
39727 PF3D7_1239200 transcription factor with AP2 domain(s) 0.12 -2.987
39572 PF3D7_1035000 U2 snRNA/tRNA pseudouridine synthase, putative 0.12 -3.08 5.4.99.12
39614 PF3D7_1119800
alternative splicing factor ASF-1, putative 0.121 -2.88
39939 PF3D7_1438500
cleavage and polyadenylation specificity factor subunit 3, putative 0.121 -3.09
39785 PF3D7_1314900
general transcription factor IIH subunit 2 0.121 -3.113
39942 PF3D7_1439700 leucine carboxyl methyltransferase, putative 0.121 -3.182 2.1.1.-
39296 PF3D7_0521500 ribosomal large subunit pseudouridylate synthase, putative 0.121 -3.016 4.2.1.70
39562 PF3D7_1030000 transcription elongation factor SPT4, putative 0.121 -2.724
39953 PF3D7_1443300 U6 snRNA-associated Sm-like protein LSm5, putative 0.121 -3.185
39315 PF3D7_0602100 ATP-dependent RNA helicase, mtr4 0.122 -3.01 3.6.4.13
39794 PF3D7_1319300 tRNA (guanine(26)-n(2))-dimethyltransferase, putative 0.122 -3.02 2.1.1.32
39955 PF3D7_1443800 pre-mrna-splicing factor cwc24 0.122 -3.101
39949 PF3D7_1441900 RNA polymerase II transcription factor B subunit 5, putative 0.122 0 2.7.7.6
39905 PF3D7_1414800 small nuclear ribonucleoprotein-associated protein B, putative 0.122 -3.272
39217 PF3D7_0323500 survival motor neuron-like protein 0.122 -2.931 3.1.31.1
39766 PF3D7_1307100 U3 small nucleolar RNA-associated protein 6, putative 0.122 -2.886
39442 PF3D7_0821300 ATP-dependent RNA helicase  dhx36, putative 0.123 -2.945 3.6.4.13
39249 PF3D7_0422500 pre-mRNA-splicing helicase BRR2, putative 0.123 -2.951 3.6.4.13
39640 PF3D7_1133800
RNA (uracil-5-)methyltransferase, putative 0.123 -3.149 2.1.1.35
39438 PF3D7_0818400 rRNA-processing protein fcf1, putative 0.123 -2.918
39768 PF3D7_1307900 tripartite motif protein, putative 0.123 -3.039
39476 PF3D7_0912800 tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6, putative 0.123 -3.103 2.1.1.220
39124 PF3D7_0103000 vacuolar protein sorting-associated protein VTA1, putative 0.123 -3.123
39712 PF3D7_1232900 nucleotidyltransferase, putative 0.124 -3.025 2.7.7.-
39182 PF3D7_0302000 pre-mRNA-splicing factor PRP46, putative 0.124 -3.009
39772 PF3D7_1309300 U4/U6 small nuclear ribonucleoprotein PRP3, putative 0.124 -3.064
39462 PF3D7_0903400 ATP-dependent RNA helicase ddx60, putative 0.125 -3.11 3.6.4.13
39536 PF3D7_1011800 PRE-binding protein 0.125 -2.928
39306 PF3D7_0525600 RNA methyltransferase, putative 0.125 -3.225 2.1.1.56
39970 PF3D7_1454100 tRNA intron endonuclease, putative 0.125 -3.121 3.1.27.9
39601 PF3D7_1111000 RNA cytosine c(5)-methyltransferase nsun2 0.125 -2.971 2.1.1.29
39227 PF3D7_0409100 U4/U6 small nuclear ribonucleoprotein PRP31, putative 0.125 -3.04
39161 PF3D7_0218400 ATPdependent RNA helicase ddx47, putative 0.126 -3.044 3.6.4.13
39958 PF3D7_1445200 ATP-dependent RNA helicase MAK5, putative 0.126 -2.897 3.6.4.13
39552 PF3D7_1021500 ATP-dependent RNA helicase ROK1, putative 0.126 -3.27 3.6.4.13
39932 PF3D7_1434000
CCR4-associated factor 16, putative 0.126 -3.1
39377 PF3D7_0708100 DNA-directed RNA polymerases I, II, and III subunit RPABC5, putative 0.126 0 2.7.7.6
39411 PF3D7_0729500 n6-adenosine-methyltransferase mt-a70; METTL3 0.126 -3.166 2.1.1.62
39367 PF3D7_0705000 mrna cap guanine-n7 methyltransferase, putative 0.126 -2.834 2.1.1.56
39347 PF3D7_0625600 poly(A) polymerase PAP, putative 0.126 -2.835 2.7.7.19
39564 PF3D7_1030700 RNA methyltransferase, putative 0.126 -2.759 2.1.1.34
39273 PF3D7_0509400
RNA polymerase I 0.126 -2.929 2.7.7.6
40006 PF3D7_1474500 splicing factor 3A subunit 1, putative 0.126 -2.958
39748 PF3D7_1249800 THO complex subunit 2, putative 0.126 -3.089
39259 PF3D7_0502100 HCNGP-like protein 0.127 -2.998
39343 PF3D7_0621300
mRNA-binding protein puf3 0.127 -3.04
39162 PF3D7_0218700 pre-mRNA-processing protein 45, putative 0.127 -3.175
39708 PF3D7_1227900
RNA pseudouridylate synthase, putative 0.127 -2.986 4.2.1.70
39497 PF3D7_0924700 splicing factor 3A subunit 3, putative 0.127 -3.116
39667 PF3D7_1203500
threonylcarbamoyl-AMP synthase, putative 0.128 -2.983 2.7.7.87
39332 PF3D7_0610900 transcription elongation factor SPT5, putative 0.128 -2.889
39437 PF3D7_0817100 tRNA modification GTPase, putative 0.128 -2.835
39525 PF3D7_1005100 U3 small nucleolar RNA-associated protein 25, putative 0.128 -3.14
39656 PF3D7_1143300 DNA-directed RNA polymerases I and III subunit RPAC1, putative 0.129 -2.889 2.7.7.6
39773 PF3D7_1309500 H/ACA ribonucleoprotein complex subunit 1, putative 0.129 -3.568
39689 PF3D7_1217200 multiple rna-binding domain-containing protein 1 0.129 -2.915
39494 PF3D7_0922700 pre-mRNA-splicing factor 18, putative 0.129 -3.198
39317 PF3D7_0603100 RNA-binding protein, putative 0.129 -2.971
39360 PF3D7_0631000 tetratricopeptide repeat protein, putative 0.129 -2.938
39523 PF3D7_1003800 U5 small nuclear ribonucleoprotein component, putative 0.129 -3.047
39981 PF3D7_1459000
ATP-dependent RNA helicase DBP5 0.13 -2.887 3.6.4.13
39591 PF3D7_1106600 DEAD/DEAH box helicase, putative 0.13 -2.958 3.6.4.13
39864 PF3D7_1357700 U3 small nucleolar RNA-associated protein 21, putative 0.13 -2.866
39224 PF3D7_0405000 ATP-dependent RNA helicase ddx51, putative 0.131 -3.135 3.6.4.13
39833 PF3D7_1343900 U4/U6 small nuclear ribonucleoprotein PRP4, putative 0.131 -2.85
39491 PF3D7_0920900 U4/U6 snRNA-associated-splicing factor, putative 0.131 -2.956
39779 PF3D7_1311200 transducin beta-like protein tbl1, putative 0.132 -3.147
39908 PF3D7_1415200 DNA-directed RNA polymerases I and III subunit RPAC2, putative 0.132 0 2.7.7.6
39723 PF3D7_1238300 pre-mRNA-splicing factor CWC22, putative 0.132 -2.84
39998 PF3D7_1469300 pre-rRNA-processing protein pno1, putative 0.132 -3.204
39681 PF3D7_1213700 DNA-directed RNA polymerases I, II, and III subunit RPABC3, putative 0.133 -3.188 2.7.7.6