Sulfenylated proteins  identified  by  mass  spectrometry
Schipper S, Wu H, Furdui CM, Poole LB, Delahunty CM, Park R, Yates JR 3rd, Becker K, Przyborski JM. Identification of sulfenylation patterns in trophozoite stage Plasmodium falciparum using a non-dimedone based probe. Mol Biochem Parasitol. 2021 26:111362. doi: Epub ahead of print. PMID: 33513391.
PfID Annotation Sequence coverage in each replicate a Sulfenic acid site b Protein nitrosylated [16] c Protein glutathionylated [15] d EC# Transcript
1 PF3D7_0511800
inositol-3-phosphate synthase 69.9% / 76.0% / 71.9% C560 + + 5.5.1.4
2 PF3D7_0513300
purine nucleoside phosphorylase 71.4% / 69.8% / 82.0% C71              C141           C208 + + 2.4.2.1
3 PF3D7_0608800
ornithine aminotransferase 65.9% / 54.1% / 40.6% C63 + + 2.6.1.13
4 PF3D7_0621200
pyridoxine biosynthesis protein PDX1 63.1% / 62.5% / 63.1% C16             C177 + +
5 PF3D7_0810800
hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase 43.2% / 51.4% / 42.1% C473 + 2.5.1.15
6 PF3D7_0918900
gamma-glutamylcysteine synthetase 22.8% / 29.6% / 32.8% C1036 + C1038 + 6.3.2.2
7 PF3D7_0922200
S-adenosylmethionine synthetase 71.9% / 73.1% / 68.4% C264 + C113 + 2.5.1.6
8 PF3D7_0922600 glutamine synthetase, putative 57.3% / 61.7% / 50.3% C66              C346           C479 6.3.1.2
9 PF3D7_0926700
glutamine-dependent NAD(+) synthetase, putative 24.7% / 22.8% / 11.9% C91 + 6.3.5.1
10 PF3D7_1343000
phosphoethanolamine N-methyltransferase 82.0% / 82.0% / 86.1% C70                C87                   C161 + + 2.1.1.103
11 PF3D7_1354500
adenylosuccinate synthetase 46.4% / 52.9% / 35.5% C283            C396 + 6.3.4.4
Cell cycle/Proliferation
12 PF3D7_0511000
translationally-controlled tumor protein homolog 60.2% / 60.2% / 67.3% C14 + C14 +
13 PF3D7_0619400
cell division cycle protein 48 homologue, putative 56.2% / 61.4% / 67.4% C418 + C425     C425           C575 + C695 +
Cellular redox homeostasis
14 PF3D7_0802200
1-cys peroxiredoxin 58.6% / 61.4% / 45.0% C101 + C184 + 1.11.1.15
16 PF3D7_0923800
thioredoxin reductase 44.7% / 34.2% / 39.7%    51.0% / 39.0% / 45.3% C422 + 1.8.1.9
17 PF3D7_1438900
thioredoxin peroxidase 1 79.0% / 86.2% / 63.6% C170 + + 1.11.1.15
Cytoskeleton
18 PF3D7_0422300
alpha tubulin 2, transcript expression profile microgametocyte 33.6% / 35.8% / 35.8%   C376
19 PF3D7_0903700
alpha tubulin 1 45.3% / 49.0% / 43.9% C376 + C65
20 PF3D7_1008700
tubulin beta chain 42.9% / 50.8% / 43.8% C127             C129 +
21 PF3D7_1473300 ckk domain-containing protein, putative X / 1.4% / 1.4% C456
DNA and RNA metabolism and processing
22 PF3D7_0624600
iswi chromatin-remodeling complex atpase 22.6% / 25.7% / 23.3% C147 + C150* + 3.6.4.12
23 PF3D7_0711500 regulator of chromosome condensation, putative 30.1% / 24.9% / 30.5% C66
24 PF3D7_0717700
serine--tRNA ligase, putative 45.1% / 60.1% / 51.0% C247 + + 6.1.1.11
25 PF3D7_0812500 RNA-binding protein, putative 7.1% / 8.5% / 6.4% C182' +
26 PF3D7_1224300
polyadenylate-binding protein 1, putative 40.6% / 50.3% / 48.9% C410 + C234 +
27 PF3D7_1346300
DNA/RNA-binding protein Alba 2 51.2% / 46.0% / 46.4% C133 + +
28 PF3D7_1347500
DNA/RNA-binding protein Alba 4 70.7% / 77.4% / 74.2% C63              C286 + +
29 PF3D7_1461900
valine--tRNA ligase, putative 45.0% / 53.1% / 45.2% C428 + 6.1.1.9
Glycolysis
30 PF3D7_0626800
pyruvate kinase 60.3% / 56.6% / 58.7% C222             C302                C343                    C433 + + 2.7.1.40
31 PF3D7_0915400
ATP-dependent 6-phosphofructokinase 55.7% / 54.5% / 53.2% C645             C1321                  C1038 + C777 + 2.7.1.11
32 PF3D7_1015900
enolase 81.8% / 83.9% / 87.2% C157                C370 + + 4.2.1.11
33 PF3D7_1324900
L-lactate dehydrogenase 74.4% / 72.5% / 71.8% C59             C260 + + 1.1.1.27
34 PF3D7_1439900
triosephosphate isomerase 72.2% / 73.8% / 76.2% C217 + + 5.3.1.1
35 PF3D7_1444800
fructose-bisphosphate aldolase 84.0% / 83.2% / 82.1% C247 + 4.1.2.13
36 PF3D7_1462800
glyceraldehyde-3-phosphate dehydrogenase 78.9% / 81.9% / 81.6% C58              C157                  C250 + + 1.2.1.12
Kinase/ Signal transduction
37 PF3D7_0818200
14-3-3 protein 65.6% / 63.0% / 61.1% C41 + +
38 PF3D7_0826700
receptor for activated c kinase 57.6% / 58.5% / 60.4% C162           C177              C216                C384 + +
39 PF3D7_1103700
casein kinase II beta chain 29.4% / 34.3% / 27.3% C122 + 2.7.11.1
40 PF3D7_1124600
ethanolamine kinase 39.5% / 53.2% / 57.4% C283 + + 2.7.1.82
41 PF3D7_1144500 SUMO-activating enzyme subunit 1 28.1% / 25.7% / 29.9% C297
42 PF3D7_1242800
rab specific GDP dissociation inhibitor 54.5% / 64.7% / 56.0% C250 +
43 PF3D7_1414400
serine/threonine protein phosphatase PP1 50.3% / 58.2% / 53.3% C169 3.1.3.16
Miscellaneous
44 PF3D7_0406400
cytosolic glyoxalase II 45.6% / 42.6% / 42.2% C4 + + 3.1.2.6
45 PF3D7_1012400
hypoxanthine-guanine phosphoribosyltransferase 72.7% / 81.8% / 73.2% C134 + + 2.4.2.8
46 PF3D7_1033400
haloacid dehalogenase-like hydrolase 56.9% / 55.9% / 63.5% C92 + 3.8.1.-
47 PF3D7_1311900
V-type proton ATPase catalytic subunit A 72.5% / 79.5% / 67.3% C526 + + 3.6.3.14
48 PF3D7_1406300
glycerophosphodiester phosphodiesterase 67.4% / 68.2% / 53.9% C91 + 3.1.4.46
Pathogenesis / Evasion / Protein export / Antigen
49 PF3D7_0929400
high molecular weight rhoptry protein 2 61.3% / 60.4% / 62.6% C1344/C1344' + +
50 PF3D7_0930300
merozoite surface protein 1 41.5% / 60.6% / 63.7% C173 + +
51 PF3D7_1028700
merozoite TRAP-like protein X / 8.4% / 26.3% C47
52 PF3D7_1029600
adenosine deaminase 68.7% / 80.7% / 70.3% C173             C281 + + 3.5.4.4
53 PF3D7_1126000
threonine--tRNA ligase 43.8% / 47.0% / 40.4% C428           C589           C767 + 6.1.1.3
54 PF3D7_1229400
macrophage migration inhibitory factor 94.8% / 95.7% / 95.7% C3/C3'            C4/C4'             C3+C4 + + 5.3.2.1
Protein / hemoglobin degradation
55 PF3D7_0207600
serine repeat antigen 5 48.4% / 58.8% / 57.4% C672 + + 3.4.22.-
56 PF3D7_1115400
cysteine proteinase falcipain 3 42.9% / 48.4% / 41.9% C331 + + 3.4.22.-
57 PF3D7_1118300 insulinase, putative 36.6% / 31.3% / 31.9% C322           C1364 + + 3.4.24.56
58 PF3D7_1225800
ubiquitin-activating enzyme E1 48.6% / 51.2% / 46.5% C641 + 6.2.1.45
59 PF3D7_1311800
M1-family alanyl aminopeptidase 76.9% / 80.0% / 78.9% C140           C617 + + 3.4.11.2
60 PF3D7_1360800
falcilysin 51.4% / 57.3% / 61.3% C438 + + 3.4.24.-
61 PF3D7_1407800
plasmepsin IV 47.0% / 48.8% / 43.0% C370 + + 3.4.23.-
62 PF3D7_1446200
M17 leucyl aminopeptidase 59.0% / 53.9% / 55.4% C121 + + 3.4.11.1
Protein degradation
63 PF3D7_0317000 proteasome subunit alpha type-3, putative 46.0% / 42.9% / 39.3% C222 + 3.4.25.1
64 PF3D7_0727400
proteasome subunit alpha type-5, putative 82.8% / 75.0% / 82.8% C76                C112 + + 3.4.25.1
65 PF3D7_0807500 proteasome subunit alpha type-6, putative 81.9% / 77.3% / 68.8% C172 + 3.4.25.1
66 PF3D7_0932300
M18 aspartyl aminopeptidase 52.6% / 52.3% / 49.5% C226 + 3.4.11.21
67 PF3D7_1248900
26S protease regulatory subunit 8, putative 33.1% / 32.2% / 35.6% C391 3.6.4.8
68 PF3D7_1306400
26S protease regulatory subunit 10B, putative; 26S proteasome AAA-ATPase subunit RPT4, putative 35.6% / 42.2% / 38.9% C111' 3.6.4.8
69 PF3D7_1402300
26S proteasome regulatory subunit RPN6 20.6% / 23.0% / 16.2% C377
Protein folding
70 PF3D7_0214000
T-complex protein 1 subunit theta 48.2% / 51.3% / 40.8% C184           C499
71 PF3D7_0320300 T-complex protein 1 subunit epsilon 40.4% / 25.6% / 32.7% C458 +
72 PF3D7_0322000
peptidyl-prolyl cis-trans isomerase 64.3% / 49.1% / 49.1% C69 + C168 + 5.2.1.8
73 PF3D7_0527500
Hsc70-interacting protein 29.7% / 30.8% / 23.4% C17                 C187 + C187 +
74 PF3D7_0627500
protein DJ-1 86.2% / 86.2% / 81.5% C85                C106/C106' + +
75 PF3D7_0708400
heat shock protein 90 58.3% / 63.0% / 59.9% C393            C616 + +
76 PF3D7_0708800
heat shock protein 110 50.2% / 57.4% / 59.9% C265               C606 + +
77 PF3D7_0818900
heat shock protein 70 77.3% / 65.6% / 68.5% C28             C583 + +
78 PF3D7_0827900
protein disulfide isomerase 68.1% / 69.8% / 75.2% C384               C387 + + 5.3.4.1
79 PF3D7_1118200 heat shock protein 90, putative 43.2% / 48.6% / 39.2% C556 +
80 PF3D7_1344200 endoplasmic reticulum chaperone grp170 62.4% / 72.4% / 73.6% C23 + +
Protein transport
81 PF3D7_0210000 secretory complex protein 61 gamma subunit 34.6% / 34.6% / 34.6% C19
82 PF3D7_0214100
protein transport protein SEC31 25.8% / 33.6% / 29.4% C309
83 PF3D7_0524000 karyopherin beta 59.1% / 60.1% / 52.9% C212            C452 +
84 PF3D7_1361100
protein transport protein Sec24A 22.6% / 28.5% / 29.0% C251
Transcription
85 PF3D7_1011800 PRE-binding protein 49.0% / 46.8% / 51.8% C505 + +
Translation
86 PF3D7_0309600
60S acidic ribosomal protein P2 92.0% / 92.0% / 82.1% C12 + +
87 PF3D7_1103100
60S acidic ribosomal protein P1, putative 85.6% / 83.1% / 83.1% C19 +
88 PF3D7_1204300
eukaryotic translation initiation factor 5A 51.6% / 51.6% / 54.0% C73 +C73      +C148 +
89 PF3D7_1302800 40S ribosomal protein S7, putative 48.5% / 22.2% / 33.0% C23
90 PF3D7_1323400 60S ribosomal protein L23 31.6% / 25.3% / 19.5% C134
91 PF3D7_1338300 elongation factor 1-gamma, putative 46.0% / 55.2% / 54.3% C70 + +
92  PF3D7_1357100 68.8% / 63.9% / 67.5% C356 + +
93 PF3D7_1357000
elongation factor 1-alpha 68.8% / 63.9% / 67.5% C356 + +
94 PF3D7_1434600
methionine aminopeptidase 2 33.9% / 30.4% / 25.3% C447 3.4.11.18
95 PF3D7_1438000 eukaryotic translation initiation factor eIF2A, putative 36.6% / 36.9% / 31.6% C176 +
96 PF3D7_1451100
elongation factor 2 59.3% / 63.3% / 67.1% C130            C362                 C653                  C725                 C786 + C565 +
97 PF3D7_1468700
eukaryotic initiation factor 4A helicase 45 67.3% / 67.3% / 63.8% C291 + + 3.6.4.12
Unknown
98 PF3D7_0321800 WD repeat-containing protein, putative X / X / 0.6% C2321
99 PF3D7_0811400 conserved protein, unknown function 45.9% / 54.1% / 43.9% C517 +
100 PF3D7_0813300 npl domain-containing protein, putative 32.4% / 32.4% / 32.4% C61 + +
101 PF3D7_1023900
SNF2 helicase, putative chromodomain-helicase-DNA-binding protein 1 homolog, putative 16.0% / 18.4% / 19.4% C1375 + + 3.6.4.12
102 PF3D7_1205600 tetratricopeptide repeat protein, putative 28.5% / 37.2% / 27.6% C213 + +
PF3D7_1361800 glideosome-associated connector 42.7% / 39.8% / 41.5% C821           C1337             C1549
a) Indicates the percentage of a protein’s sequence in one of three independently conducted experiments which has been covered in mass spectrometry analysis.    b) Cysteines which were identified as being sulfenylated due to a mass shift of C = SO-NEM (+141.0426); C* = SO-BCN-Bio1 (+392.177), and their position in a protein’s amino sequence are listed. “C+C” indicates two cysteines in the same peptide sequence which were found to be simultaneously sulfenylated. c) “+” indicates proteins which were also identified by Wang et al. as being S-nitrosated. If available, the modified cysteine is given. Proteins found to be non-specifically enriched were subtracted [16]. d) “+” indicates proteins which were also identified by Kehr et al. as being S-glutathionylated. Proteins found to be non-specifically enriched were subtracted [15]. e) SP, signal peptide; AP, apicoplast; PV, parasitophorous vacuole; PM, plasma membrane; ER, endoplasmic reticulum; FV, food vacuole; Rhop, rhoptry. Annotation based on PlasmoDB/SIgnalP analyses.
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